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Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Islands

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Abstract

Rhizobacteria with properties related to plant growth-promotion were isolated from the rhizosphere of the perennial legume Chamaecytisus proliferus ssp. proliferus var. palmensis (tagasaste) growing in field conditions. Samples were collected in two localities of the Tenerife Island: La Laguna and El Tanque, NE and NW at 600 and 1000 meters above sea level, respectively, and in two seasons, winter and summer. The strains were isolated by using culture dependent procedures, and identified by phenotypic (culturable and biochemical) and genotypic (ERIC-PCR fingerprinting) features. The rhizosphere isolates formed a diverse community of mainly Gram-negative bacteria, with members of genera Pseudomonas, Burkholderia and Sphingomonas being predominant. A high level of selectivity was found in the rhizosphere environment as compared to the non-rhizosphere soil where Gram-positives were more abundant. Species richness (number of species) and species abundance were related to the sampling season and the locality, thus, samples obtained in winter at both sites had larger counts than samples obtained in summer, and the higher species richness was found in La Laguna. The species Pseudomonas fluorescens showed the highest number of properties related to plant growth promotion (PGP): 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, phytohormone production, nitrogen fixation, fungal growth inhibition and cyanogenesis, thus it seems to be the most suitable microorganism to be tested in PGP-field experiments.

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Authors and Affiliations

  1. Departamento de Microbiología y Biología Celular. Facultad de Farmacia, Universidad de La Laguna, 38071, La Laguna. Tenerife. Canary Islands, Spain

    Javier Donate-Correa, Milagros León-Barrios & Ricardo Pérez-Galdona

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  1. Javier Donate-Correa
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  2. Milagros León-Barrios
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  3. Ricardo Pérez-Galdona
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Correspondence to Ricardo Pérez-Galdona.

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Donate-Correa, J., León-Barrios, M. & Pérez-Galdona, R. Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Islands. Plant Soil 266, 261–272 (2005). https://doi.org/10.1007/s11104-005-0754-5

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