Plant Molecular Biology

, Volume 87, Issue 4, pp 395–411

Identification and characterization of plant-specific NAC gene family in canola (Brassica napus L.) reveal novel members involved in cell death

  • Boya Wang
  • Xiaohua Guo
  • Chen Wang
  • Jieyu Ma
  • Fangfang Niu
  • Hanfeng Zhang
  • Bo Yang
  • Wanwan Liang
  • Feng Han
  • Yuan-Qing Jiang
Article

DOI: 10.1007/s11103-015-0286-1

Cite this article as:
Wang, B., Guo, X., Wang, C. et al. Plant Mol Biol (2015) 87: 395. doi:10.1007/s11103-015-0286-1

Abstract

NAC transcription factors are plant-specific and play important roles in plant development processes, response to biotic and abiotic cues and hormone signaling. However, to date, little is known about the NAC genes in canola (or oilseed rape, Brassica napus L.). In this study, a total of 60 NAC genes were identified from canola through a systematical analysis and mining of expressed sequence tags. Among these, the cDNA sequences of 41 NAC genes were successfully cloned. The translated protein sequences of canola NAC genes with the NAC genes from representative species were phylogenetically clustered into three major groups and multiple subgroups. The transcriptional activities of these BnaNAC proteins were assayed in yeast. In addition, by quantitative real-time RT-PCR, we further observed that some of these BnaNACs were regulated by different hormone stimuli or abiotic stresses. Interestingly, we successfully identified two novel BnaNACs, BnaNAC19 and BnaNAC82, which could elicit hypersensitive response-like cell death when expressed in Nicotiana benthamiana leaves, which was mediated by accumulation of reactive oxygen species. Overall, our work has laid a solid foundation for further characterization of this important NAC gene family in canola.

Keywords

Abiotic stress Brassica napus Cell death NAC ROS Transcription factors 

Supplementary material

11103_2015_286_MOESM1_ESM.pdf (220 kb)
Supplementary material 1 (PDF 219 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Boya Wang
    • 1
  • Xiaohua Guo
    • 1
  • Chen Wang
    • 1
  • Jieyu Ma
    • 1
  • Fangfang Niu
    • 1
  • Hanfeng Zhang
    • 1
  • Bo Yang
    • 1
  • Wanwan Liang
    • 1
  • Feng Han
    • 1
  • Yuan-Qing Jiang
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas, and College of Life SciencesNorthwest A & F UniversityYanglingChina