Plant Molecular Biology

, Volume 85, Issue 6, pp 613–625

Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex

  • Wenxin Tang
  • Yonghui He
  • Lili Tu
  • Maojun Wang
  • Yang Li
  • Yong-Ling Ruan
  • Xianlong Zhang
Article

DOI: 10.1007/s11103-014-0208-7

Cite this article as:
Tang, W., He, Y., Tu, L. et al. Plant Mol Biol (2014) 85: 613. doi:10.1007/s11103-014-0208-7

Abstract

Cotton fiber is a single cell that differentiates from the ovule epidermis and undergoes synchronous elongation with high secretion and growth rate. Apart from economic importance, cotton fiber provides an excellent single-celled model for studying mechanisms of cell-growth. Annexins are Ca2+- and phospholipid-binding proteins that have been reported to be localized in multiple cellular compartments and involved in control of vesicle secretions. Although several annexins have been found to be highly expressed in elongating cotton fibers, their functional roles in fiber development remain unknown. Here, 14 annexin family members were identified from the fully sequenced diploid G. raimondii (D5 genome), half of which were expressed in fibers of the cultivated tetraploid species G. hirsutum (cv. YZ1). Among them, GhAnn2 from the D genome of the tetraploid species displayed high expression level in elongating fiber. The expression of GhAnn2 could be induced by some phytohormones that play important roles in fiber elongation, such as IAA and GA3. RNAi-mediated down-regulation of GhAnn2 inhibited fiber elongation and secondary cell wall synthesis, resulting in shorter and thinner mature fibers in the transgenic plants. Measurement with non-invasive scanning ion-selective electrode revealed that the rate of Ca2+ influx from extracellular to intracellular was decreased at the fiber cell apex of GhAnn2 silencing lines, in comparison to that in the wild type. These results indicate that GhAnn2 may regulate fiber development through modulating Ca2+ fluxes and signaling.

Keywords

Gossypium hirsutum (cotton)GhAnn2Fiber elongationFiber secondary cell wall synthesisCalcium signaling

Supplementary material

11103_2014_208_MOESM1_ESM.pdf (1007 kb)
Supplementary material 1 (PDF 1007 kb)
11103_2014_208_MOESM2_ESM.xls (121 kb)
Supplementary material 2 (XLS 121 kb)
11103_2014_208_MOESM3_ESM.doc (38 kb)
Supplementary material 3 (DOC 38 kb)
11103_2014_208_MOESM4_ESM.doc (142 kb)
Supplementary material 4 (DOC 141 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Wenxin Tang
    • 1
  • Yonghui He
    • 1
  • Lili Tu
    • 1
  • Maojun Wang
    • 1
  • Yang Li
    • 1
  • Yong-Ling Ruan
    • 2
  • Xianlong Zhang
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Department of Biology, School of Environmental and Life SciencesThe University of NewcastleNewcastleAustralia