Plant Molecular Biology

, Volume 83, Issue 3, pp 177–189

BAC-end sequences analysis provides first insights into coffee (Coffea canephora P.) genome composition and evolution

  • Alexis Dereeper
  • Romain Guyot
  • Christine Tranchant-Dubreuil
  • François Anthony
  • Xavier Argout
  • Fabien de Bellis
  • Marie-Christine Combes
  • Frederick Gavory
  • Alexandre de Kochko
  • Dave Kudrna
  • Thierry Leroy
  • Julie Poulain
  • Myriam Rondeau
  • Xiang Song
  • Rod Wing
  • Philippe Lashermes
Article

DOI: 10.1007/s11103-013-0077-5

Cite this article as:
Dereeper, A., Guyot, R., Tranchant-Dubreuil, C. et al. Plant Mol Biol (2013) 83: 177. doi:10.1007/s11103-013-0077-5

Abstract

Coffee is one of the world’s most important agricultural commodities. Coffee belongs to the Rubiaceae family in the euasterid I clade of dicotyledonous plants, to which the Solanaceae family also belongs. Two bacterial artificial chromosome (BAC) libraries of a homozygous doubled haploid plant of Coffea canephora were constructed using two enzymes, HindIII and BstYI. A total of 134,827 high quality BAC-end sequences (BESs) were generated from the 73,728 clones of the two libraries, and 131,412 BESs were conserved for further analysis after elimination of chloroplast and mitochondrial sequences. This corresponded to almost 13 % of the estimated size of the C. canephora genome. 6.7 % of BESs contained simple sequence repeats, the most abundant (47.8 %) being mononucleotide motifs. These sequences allow the development of numerous useful marker sites. Potential transposable elements (TEs) represented 11.9 % of the full length BESs. A difference was observed between the BstYI and HindIII libraries (14.9 vs. 8.8 %). Analysis of BESs against known coding sequences of TEs indicated that 11.9 % of the genome corresponded to known repeat sequences, like for other flowering plants. The number of genes in the coffee genome was estimated at 41,973 which is probably overestimated. Comparative genome mapping revealed that microsynteny was higher between coffee and grapevine than between coffee and tomato or Arabidopsis. BESs constitute valuable resources for the first genome wide survey of coffee and provide new insights into the composition and evolution of the coffee genome.

Keywords

Comparative genomicsCoffeaGenomeBAC libraryTransposable elementsMicrosatellites

Supplementary material

11103_2013_77_MOESM1_ESM.doc (234 kb)
Supplementary material 1 (DOC 234 kb)
11103_2013_77_MOESM2_ESM.txt (4.7 mb)
Supplementary material 2 (TXT 4838 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Alexis Dereeper
    • 1
  • Romain Guyot
    • 2
  • Christine Tranchant-Dubreuil
    • 2
  • François Anthony
    • 1
  • Xavier Argout
    • 3
  • Fabien de Bellis
    • 3
  • Marie-Christine Combes
    • 1
  • Frederick Gavory
    • 4
  • Alexandre de Kochko
    • 2
  • Dave Kudrna
    • 5
  • Thierry Leroy
    • 3
  • Julie Poulain
    • 4
  • Myriam Rondeau
    • 1
  • Xiang Song
    • 5
  • Rod Wing
    • 5
  • Philippe Lashermes
    • 1
  1. 1.Institut de Recherche pour le Développement (IRD)UMR RPB (CIRAD, IRD, UM2)Montpellier Cedex 5France
  2. 2.Institut de Recherche pour le Développement (IRD)UMR DIADE (CIRAD, IRD, UM2)Montpellier Cedex 5France
  3. 3.Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)UMR AGAPMontpellierFrance
  4. 4.Commissariat à l’Energie Atomique (CEA)Institut de GénomiqueEvryFrance
  5. 5.Arizona Genomics Institute, School of Plant SciencesUniversity of ArizonaTucsonUSA