Article

Plant Molecular Biology

, Volume 81, Issue 1, pp 57-69

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Functional analysis of splice variant expression of MADS AFFECTING FLOWERING 2 of Arabidopsis thaliana

  • Sarah Marie RosloskiAffiliated withDepartment of Biology, Western University
  • , Anandita SinghAffiliated withDepartment of Molecular Biology, Max-Planck Institute for Developmental BiologyDepartment of Biotechnology, Faculty of Applied Science, TERI University
  • , Sathya Sheela JaliAffiliated withDepartment of Biology, Western University
  • , Sureshkumar BalasubramanianAffiliated withDepartment of Molecular Biology, Max-Planck Institute for Developmental BiologySchool of Biological Sciences, Monash University
  • , Detlef WeigelAffiliated withDepartment of Molecular Biology, Max-Planck Institute for Developmental Biology
  • , Vojislava GrbicAffiliated withDepartment of Biology, Western University Email author 

Abstract

The MADS-AFFECTING FLOWERING 2 (MAF2) gene of Arabidopsis thaliana has been characterized as a repressor of flowering. The molecular basis of MAF2 gene function and role of alternative MAF2 transcripts in flowering time modulation is not understood. MAF2 splice variant expression was quantified in cold-acclimated plants by quantitative RT-PCR. Cold influenced the abundance of splice variants and prompted a functional study of splice forms. Individual variants were overexpressed in the Col background and were assayed for their ability to delay flowering. Overexpression of MAF2 variants 2 and 4 had limited effect on flowering time. Overexpression of MAF2 splice variant 1 resulted in early flowering and affected the expression of the endogenous MAF2 gene and its paralogues, confounding functional assessment. In the Ll-2 Arabidopsis accession, a MAF2, MAF3, MAF4 and FLC null line, MAF2 var1 was consistent in its effect on reproductive delay under ambient and reduced temperatures, indicating that it acts as a repressor of flowering.

Keywords

Arabidopsis thaliana Flowering time Alternative splicing Response to cold MAF2