Plant Molecular Biology

, Volume 80, Issue 3, pp 241–253

OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice

Authors

  • Huaishun Shen
    • Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research CenterChinese Academy of Fishery Sciences
    • National Engineering Research Center for Molecular Crop DesignBeijing Weiming Kaituo Agriculture Biotech Co., Ltd
  • Citao Liu
    • National Engineering Research Center for Molecular Crop DesignBeijing Weiming Kaituo Agriculture Biotech Co., Ltd
    • State Key Laboratory of Plant Genomics and Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental BiologyChinese Academy of Sciences
  • Yi Zhang
    • National Engineering Research Center for Molecular Crop DesignBeijing Weiming Kaituo Agriculture Biotech Co., Ltd
  • Xiuping Meng
    • National Engineering Research Center for Molecular Crop DesignBeijing Weiming Kaituo Agriculture Biotech Co., Ltd
  • Xin Zhou
    • Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research CenterChinese Academy of Fishery Sciences
  • Chengcai Chu
    • State Key Laboratory of Plant Genomics and Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental BiologyChinese Academy of Sciences
    • National Engineering Research Center for Molecular Crop DesignBeijing Weiming Kaituo Agriculture Biotech Co., Ltd
Article

DOI: 10.1007/s11103-012-9941-y

Cite this article as:
Shen, H., Liu, C., Zhang, Y. et al. Plant Mol Biol (2012) 80: 241. doi:10.1007/s11103-012-9941-y

Abstract

Both the WRKY transcription factor (TF) and MAP kinases have been shown to regulate gene expression in response to biotic and abiotic stresses in plants. Several reports have shown that WRKY TFs may function downstream of MAPK cascades. Here, we have shown that OsWRKY30 interacted with OsMPK3, OsMPK4, OsMPK7, OsMPK14, OsMPK20-4, and OsMPK20-5, and could be phosphorylated by OsMPK3, OsMPK7, and OsMPK14. Overexpression of OsWRKY30 in rice dramatically increased drought tolerance. Overexpression of OsWRKY30AA, in which all SP (serine residue followed by proline residue) sites were replaced by AP (A, alanine), resulted in no improvement in drought tolerance. In addition, the function of transcriptional activation of OsWRKY30 was impaired after SP was replaced by AP. These results proved that the phosphorylation of OsWRKY30 by MAPKs was crucial in order for OsWRKY30 to perform its biological function.

Keywords

WRKYMAPKDrought tolerancePhosphorylationTranscriptional activation

Supplementary material

11103_2012_9941_MOESM1_ESM.doc (572 kb)
Supplementary material 1 (DOC 572 kb)

Copyright information

© Springer Science+Business Media B.V. 2012