Plant Molecular Biology

, 77:371

Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells

Authors

  • Eva Majerová
    • Department of Functional Genomics and Proteomics, Faculty of Science and Central European Institute of Technology (CEITEC)Masaryk University
  • Miloslava Fojtová
    • Department of Functional Genomics and Proteomics, Faculty of Science and Central European Institute of Technology (CEITEC)Masaryk University
  • Iva Mozgová
    • Department of Functional Genomics and Proteomics, Faculty of Science and Central European Institute of Technology (CEITEC)Masaryk University
  • Miroslava Bittová
    • Department of Chemistry, Faculty of ScienceMasaryk University
    • Department of Functional Genomics and Proteomics, Faculty of Science and Central European Institute of Technology (CEITEC)Masaryk University
    • Institute of BiophysicsAcademy of Sciences of the Czech Republic
Article

DOI: 10.1007/s11103-011-9816-7

Cite this article as:
Majerová, E., Fojtová, M., Mozgová, I. et al. Plant Mol Biol (2011) 77: 371. doi:10.1007/s11103-011-9816-7

Abstract

Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.

Keywords

Nicotiana tabacumCell cultureTelomereHypomethylating agentsZebularineDNA methylation

Copyright information

© Springer Science+Business Media B.V. 2011