Plant Molecular Biology

, Volume 74, Issue 3, pp 215–222

Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis

  • María José Iglesias
  • María Cecilia Terrile
  • Carlos Guillermo Bartoli
  • Sebastián D’Ippólito
  • Claudia Anahí Casalongué
Article

DOI: 10.1007/s11103-010-9667-7

Cite this article as:
Iglesias, M.J., Terrile, M.C., Bartoli, C.G. et al. Plant Mol Biol (2010) 74: 215. doi:10.1007/s11103-010-9667-7

Abstract

Auxin regulates gene expression through direct physical interaction with TIR1/AFB receptor proteins during different processes of growth and development in plants. Here we report the contribution of auxin signaling pathway to the adaptative response against abiotic stress in Arabidopsis. Phenotypic characterization of tir1/afb auxin receptor mutants indicates a differential participation of each member under abiotic stress. In particular, tir1 afb2 and tir1 afb3 mutants resulted more tolerant to oxidative stress. In addition, tir1 afb2 showed increased tolerance against salinity measured as chlorophyll content, germination rate and root elongation compared with wild-type plants. Furthermore, tir1 afb2 displayed a reduced accumulation of hydrogen peroxide and superoxide anion, as well as enhanced antioxidant enzymes activities under stress. A higher level of ascorbic acid was detected in tir1 afb2 compared with wild-type plants. Thus, adaptation to salinity in Arabidopsis may be mediated in part by an auxin/redox interaction.

Keywords

Arabidopsis thalianaAuxin signalingAbiotic stressReactive oxygen speciesAntioxidants

Abbreviations

ROS

Reactive oxygen species

MV

Methyl viologen

H2O2

Hydrogen peroxide

O2

Superoxide anion

AA

Ascorbate

DHA

Dihydroascorbate

CAT

Catalase

APX

Ascorbate peroxidase

Supplementary material

11103_2010_9667_MOESM1_ESM.pdf (23 kb)
Phenotypic analysis of single mutants, tir1 and afb2 under stress

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • María José Iglesias
    • 1
  • María Cecilia Terrile
    • 1
  • Carlos Guillermo Bartoli
    • 2
  • Sebastián D’Ippólito
    • 1
  • Claudia Anahí Casalongué
    • 1
  1. 1.Instituto de Investigaciones Biológicas, UE-CONICET-UNMdP, Facultad de Ciencias Exactas y NaturalesUniversidad Nacional de Mar del PlataMar del PlataArgentina
  2. 2.Instituto de Fisiología Vegetal. Facultad de Ciencias NaturalesUniversidad Nacional de La Plata-CCT La Plata CONICETLa PlataArgentina