Plant Molecular Biology

, Volume 73, Issue 4, pp 363–378

Promoter diversity in multigene transformation

  • Ariadna Peremarti
  • Richard M. Twyman
  • Sonia Gómez-Galera
  • Shaista Naqvi
  • Gemma Farré
  • Maite Sabalza
  • Bruna Miralpeix
  • Svetlana Dashevskaya
  • Dawei Yuan
  • Koreen Ramessar
  • Paul Christou
  • Changfu Zhu
  • Ludovic Bassie
  • Teresa Capell
Review

DOI: 10.1007/s11103-010-9628-1

Cite this article as:
Peremarti, A., Twyman, R.M., Gómez-Galera, S. et al. Plant Mol Biol (2010) 73: 363. doi:10.1007/s11103-010-9628-1

Abstract

Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.

Keywords

PromoterTransgeneMultigene transformationTranscriptional silencingConstitutiveSpatiotemporalInducible

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ariadna Peremarti
    • 1
  • Richard M. Twyman
    • 2
  • Sonia Gómez-Galera
    • 1
  • Shaista Naqvi
    • 1
  • Gemma Farré
    • 1
  • Maite Sabalza
    • 1
  • Bruna Miralpeix
    • 1
  • Svetlana Dashevskaya
    • 1
  • Dawei Yuan
    • 1
  • Koreen Ramessar
    • 1
  • Paul Christou
    • 1
    • 3
  • Changfu Zhu
    • 1
  • Ludovic Bassie
    • 1
  • Teresa Capell
    • 1
  1. 1.Departament de Producció Vegetal i Ciència Forestal, ETSEAUniversitat de LleidaLleidaSpain
  2. 2.Department of Biological SciencesUniversity of WarwickCoventryUK
  3. 3.Institució Catalana de Recerca i Estudis AvançatsBellaterraSpain