Plant Molecular Biology

, Volume 73, Issue 3, pp 251–269

Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species

Authors

  • Mingquan Ding
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Peichen Hou
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Xin Shen
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Meijuan Wang
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Shurong Deng
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Jian Sun
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Fei Xiao
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Ruigang Wang
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Xiaoyang Zhou
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Cunfu Lu
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Deqiang Zhang
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
  • Xiaojiang Zheng
    • Key Laboratory of Biological Resources Protection and Utilization in Hubei ProvinceHubei University for Nationalities
  • Zanmin Hu
    • Institute of Genetics and Developmental BiologyChinese Academy of Sciences
    • College of Biological Sciences and Technology, National Engineering Laboratory for Tree BreedingBeijing Forestry University
    • Key Laboratory of Biological Resources Protection and Utilization in Hubei ProvinceHubei University for Nationalities
Article

DOI: 10.1007/s11103-010-9612-9

Cite this article as:
Ding, M., Hou, P., Shen, X. et al. Plant Mol Biol (2010) 73: 251. doi:10.1007/s11103-010-9612-9

Abstract

Using the Affymetrix poplar genome array, we explored the leaf transcriptome of salt-tolerant Populus euphratica Oliv. and salt-sensitive P. popularis 35-44 (P. popularis) under control and saline conditions. Our objective was to clarify the genomic differences in regulating K+/Na+ and reactive oxygen species (ROS) homeostasis between the two species. Compared to P. popularis, salt-tolerant P. euphratica responses to salinity involved induction of a relatively larger number of probesets after short-term (ST) exposure to 150 mM NaCl (24 h) and relatively fewer probesets after a long-term (LT) exposure to salinity (200 mM NaCl, 28 days). Compared to P. popularis, leaves of the control P. euphratica plants exhibited a higher transcript abundance of genes related to Na+/H+ antiport (Na+/H+ antiporters, H+ pumps) and K+ uptake and transport. Notably, the expression of these genes did not decrease (with a few exceptions) during salt treatment. Regarding ROS homeostasis, P. euphratica exhibited rapid up-regulation of a variety of antioxidant enzymes after exposure to ST salinity, indicating a rapid adaptive response to salt stress. However, the effect of NaCl on transcription in P. popularis leaves was more pronounced after exposure to prolonged salinity. LT-stressed P. popularis up-regulated some genes mediating K+/Na+ homeostasis but decreased transcription of main scavengers of superoxide radicals and H2O2 except for some isoforms of a few scavengers. Mineral and ROS analyses show that NaCl induced a marked increase of leaf Na+ and H2O2 in LT-stressed plants of the two species and the effects were even more pronounced in the salt-sensitive poplar. We place the transcription results in the context of our physiological measurements to infer some implications of NaCl-induced alterations in gene expression related to K+/Na+ and ROS homeostasis.

Keywords

Affymetrix poplar genome arrayNaClPopulus euphraticaP. popularisSalt tolerance

Supplementary material

11103_2010_9612_MOESM1_ESM.doc (633 kb)
(DOC 633 kb)
11103_2010_9612_MOESM2_ESM.doc (562 kb)
(DOC 562 kb)

Copyright information

© Springer Science+Business Media B.V. 2010