Plant Molecular Biology

, Volume 73, Issue 1, pp 89–95

A role for an alternative splice variant of PIF6 in the control of Arabidopsis primary seed dormancy

  • Steven Penfield
  • Eve-Marie Josse
  • Karen J. Halliday
Article

DOI: 10.1007/s11103-009-9571-1

Cite this article as:
Penfield, S., Josse, EM. & Halliday, K.J. Plant Mol Biol (2010) 73: 89. doi:10.1007/s11103-009-9571-1

Abstract

Phytochrome interacting factor (PIF) transcription factors have been shown to be important in the regulation of seed dormancy and germination by environmental cues. Many PIF-family transcription factors are expressed in seeds but only PIF1 and SPATULA (SPT) have been tested for a role in germination control. Here we show that PIF6 is expressed strongly during seed development, and that two splice variants exist, one full length (the α form), and a second, the β form, in which a cryptic intron containing the potential DNA binding domain is spliced out, predicted to lead to the generation of a premature stop codon. Loss of PIF6 increases primary seed dormancy, whereas overexpression of the β form, but not the α form, reduce dormancy. Our data show the potential for natural splice variants of PIF transcription factors to be important in the evolution of the control of environmental signalling in plants.

Keywords

Seed germination Dormancy Phytochrome Phytochrome interacting factor Alternative splicing 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Steven Penfield
    • 1
  • Eve-Marie Josse
    • 2
  • Karen J. Halliday
    • 2
  1. 1.Centre for Novel Agricultural Products, Department of BiologyUniversity of YorkYorkUK
  2. 2.Department of Biological Sciences, CH Waddington Building, Kings BuildingsUniversity of EdinburghEdinburghUK

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