Plant Molecular Biology

, Volume 70, Issue 1, pp 173–192

Transcriptome analysis during somatic embryogenesis of the tropical monocot Elaeisguineensis: evidence for conserved gene functions in early development

Authors

  • Hsiang-Chun Lin
    • IRD, UMR DIAPC, IRD/CIRAD Palm Development Group
    • ETH ZurichInstitute of Plant Science
  • Fabienne Morcillo
    • CIRAD, UMR DIAPC, IRD/CIRAD Palm Development Group
  • Stéphane Dussert
    • IRD, UMR DIAPC
  • Christine Tranchant-Dubreuil
    • IRD, UMR DIAPC
  • James W. Tregear
    • IRD, UMR DIAPC, IRD/CIRAD Palm Development Group
    • IRD, UMR DIAPC, IRD/CIRAD Palm Development Group
Article

DOI: 10.1007/s11103-009-9464-3

Cite this article as:
Lin, H., Morcillo, F., Dussert, S. et al. Plant Mol Biol (2009) 70: 173. doi:10.1007/s11103-009-9464-3

Abstract

With the aim of understanding the molecular mechanisms underlying somatic embryogenesis (SE) in oil palm, we examined transcriptome changes that occur when embryogenic suspension cells are initiated to develop somatic embryos. Two reciprocal suppression subtractive hybridization (SSH) libraries were constructed from oil palm embryogenic cell suspensions: one in which embryo development was blocked by the presence of the synthetic auxin analogue 2,4-dichlorophenoxyacetic acid (2,4-d) in the medium (proliferation library); and another in which cells were stimulated to form embryos by the removal of 2,4-d from the medium (initiation library). A total of 1867 Expressed Sequence Tags (ESTs) consisting of 1567 potential unigenes were assembled from the two libraries. Functional annotation indicated that 928 of the ESTs correspond to proteins that have either no similarity to sequences in public databases or are of unknown function. Gene Ontology (GO) terms assigned to the two EST populations give clues to the underlying molecular functions, biological processes and cellular components involved in the initiation of embryo development. Macroarrays were used for transcript profiling the ESTs during SE. Hierarchical cluster analysis of differential transcript accumulation revealed 4 distinct profiles containing a total of 192 statistically significant developmentally regulated transcripts. Similarities and differences between the global results obtained with in vitro systems from dicots, monocots and gymnosperms will be discussed.

Keywords

Somatic embryogenesisOil palmAuxinTranscript profiling

Abbreviations

2,4-d

2,4-Dichlorophenoxyacetic acid

ARF

ADP-ribosylation factor

EST

Expressed sequence tag

GO

Gene Ontology

GST

Glutathione S-transferases

HCA

Hierarchical cluster analysis

PR

Pathogenesis-related

STM-like

Shootmeristemless-like

SEm

Somatic embryo

SE

Somatic embryogenesis

SSH

Suppression subtractive hybridization

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2009