Plant Molecular Biology

, Volume 67, Issue 4, pp 403–417

Sucrose induction of Arabidopsis miR398 represses two Cu/Zn superoxide dismutases

Article

DOI: 10.1007/s11103-008-9329-1

Cite this article as:
Dugas, D.V. & Bartel, B. Plant Mol Biol (2008) 67: 403. doi:10.1007/s11103-008-9329-1

Abstract

MicroRNAs (miRNAs) are ∼21-nt RNAs that reduce target accumulation through mRNA cleavage or translational repression. Arabidopsis miR398 regulates mRNAs encoding two copper superoxide dismutase (CSD) enzymes and a cytochrome c oxidase subunit. miR398 itself is down-regulated in response to copper and stress. Here we show that miR398 is positively regulated by sucrose, resulting in decreased CSD1 and CSD2 mRNA and protein accumulation. This sucrose regulation is maintained both in the presence and absence of physiologically relevant levels of supplemental copper. Additionally, we show that plants expressing CSD1 and CSD2 mRNAs with altered miR398 complementarity sites display increased mRNA accumulation, whereas CSD1 and CSD2 protein accumulation remain sensitive to miR398 levels, suggesting that miR398 can act as a translational repressor when target site complementarity is reduced. These results reveal a novel miR398 regulatory mechanism and demonstrate that plant miRNA targets can resist miRNA regulation at the mRNA level while maintaining sensitivity at the level of protein accumulation. Our results suggest that even in plants, where miRNAs are thought to act primarily through target mRNA cleavage, monitoring target protein levels along with target mRNA levels is necessary to fully assess the consequences of disrupted miRNA–mRNA pairing. Moreover, the limited complementarity required to maintain robust miR398-directed repression of target protein accumulation suggests that similarly regulated endogenous plant miRNA targets may have eluded detection.

Keywords

miR398microRNAPost-transcriptional regulationSucrose regulationSuperoxide dismutase

Abbreviations

CSD

Copper superoxide dismutase

FSD

Iron superoxide dismutase

miRNA

MicroRNA

PN

Plant nutrient medium

PN−m

Plant nutrient medium without micronutrients

ROS

Reactive oxygen species

SOD

Superoxide dismutase

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Biochemistry and Cell BiologyRice UniversityHoustonUSA