Plant Molecular Biology

, Volume 66, Issue 5, pp 551–563

Genome-wide allele-specific expression analysis using Massively Parallel Signature Sequencing (MPSS™) Reveals cis- and trans-effects on gene expression in maize hybrid meristem tissue

  • Mei Guo
  • Sean Yang
  • Mary Rupe
  • Bin Hu
  • David R. Bickel
  • Lane Arthur
  • Oscar Smith
Article

DOI: 10.1007/s11103-008-9290-z

Cite this article as:
Guo, M., Yang, S., Rupe, M. et al. Plant Mol Biol (2008) 66: 551. doi:10.1007/s11103-008-9290-z

Abstract

Allelic differences in expression are important genetic factors contributing to quantitative trait variation in various organisms. However, the extent of genome-wide allele-specific expression by different modes of gene regulation has not been well characterized in plants. In this study we developed a new methodology for allele-specific expression analysis by applying Massively Parallel Signature Sequencing (MPSS™), an open ended and sequencing based mRNA profiling technology. This methodology enabled a genome-wide evaluation of cis- and trans-effects on allelic expression in six meristem stages of the maize hybrid. Summarization of data from nearly 400 pairs of MPSS allelic signature tags showed that 60% of the genes in the hybrid meristems exhibited differential allelic expression. Because both alleles are subjected to the same trans-acting factors in the hybrid, the data suggest the abundance of cis-regulatory differences in the genome. Comparing the same allele expressed in the hybrid versus its inbred parents showed that 40% of the genes were differentially expressed, suggesting different trans-acting effects present in different genotypes. Such trans-acting effects may result in gene expression in the hybrid different from allelic additive expression. With this approach we quantified gene expression in the hybrid relative to its inbred parents at the allele-specific level. As compared to measuring total transcript levels, this study provides a new level of understanding of different modes of gene regulation in the hybrid and the molecular basis of heterosis.

Keywords

Allele-specific expression Cis- and trans-regulation Heterosis MPSS Zea mays L. 

Supplementary material

11103_2008_9290_MOESM1_ESM.doc (84 kb)
(DOC 83.5 kb)
11103_2008_9290_MOESM2_ESM.xls (264 kb)
(XLS 263 kb)

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mei Guo
    • 1
  • Sean Yang
    • 1
    • 2
  • Mary Rupe
    • 1
  • Bin Hu
    • 1
  • David R. Bickel
    • 1
  • Lane Arthur
    • 1
    • 3
  • Oscar Smith
    • 1
  1. 1.Pioneer Hi-Bred International, Inc., A DuPont BusinessJohnstonUSA
  2. 2.MonsantoSt. LouisUSA
  3. 3.DuPont Central Research & DevelopmentWilmingtonUSA

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