Plant Molecular Biology

, Volume 62, Issue 6, pp 927–936

A Gateway-based platform for multigene plant transformation

  • Qi-Jun Chen
  • Hai-Meng Zhou
  • Jia Chen
  • Xue-Chen Wang
Article

DOI: 10.1007/s11103-006-9065-3

Cite this article as:
Chen, QJ., Zhou, HM., Chen, J. et al. Plant Mol Biol (2006) 62: 927. doi:10.1007/s11103-006-9065-3
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Abstract

The post-genomic era offers unrivalled opportunities for genetic manipulation of polygenic traits, multiple traits, and multiple gene products. However, remaining technical hurdles make the manipulation of multiple genes in plants difficult. Here we describe a Gateway-based vector system to enable multiple transgenes to be directly linked or fused. The vector system consists of a destination vector and two special attL-flanked entry vectors each containing an attR cassette incompatible with the attL. By multiple rounds of LR recombination reactions, which we call MultiRound Gateway, multiple transgenes can be delivered sequentially and indefinitely into the Gateway-compatible destination vector through alternate use of the two special entry vectors. In our proof-of-principle experiments we have used this vector system to construct a plant transformation vector containing seven functional DNA fragments, including a screening marker gene, two reporter genes and four matrix attachment region sequences. This system provides a platform for fully realizing the potential of plant genetic manipulation.

Keywords

Gateway vectors Multigene plant transformation MultiRound Gateway 

Supplementary material

11103_2006_9065_MOESM1_ESM.doc (298 kb)
Supplementary material

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Qi-Jun Chen
    • 1
  • Hai-Meng Zhou
    • 2
  • Jia Chen
    • 1
  • Xue-Chen Wang
    • 1
  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Biological SciencesChina Agricultural UniversityBeijingChina
  2. 2.Department of Biological Science and BiotechnologyTsinghua UniversityBeijingChina

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