Plant Molecular Biology

, Volume 58, Issue 6, pp 789–807

Alfalfa Mob1-like Genes are Expressed in Reproductive Organs during Meiosis and Gametogenesis

  • Sandra Citterio
  • Emidio Albertini
  • Serena Varotto
  • Erika Feltrin
  • Marica Soattin
  • Gianpiero Marconi
  • Sergio Sgorbati
  • Margherita Lucchin
  • Gianni Barcaccia
Article

DOI: 10.1007/s11103-005-8104-9

Cite this article as:
Citterio, S., Albertini, E., Varotto, S. et al. Plant Mol Biol (2005) 58: 789. doi:10.1007/s11103-005-8104-9

Abstract

Mps-one-binder (Mob) proteins play an important role in chromosome separation and cell plate formation in yeast. We cloned two Mob1-like genes from alfalfa (Medicago sativa L.) and show that one gene is constitutively expressed while the other is expressed only in flower buds during sporogenesis and gametogenesis. For the analysis of gene expression during reproduction in alfalfa wild-types and apomeiotic mutants, a specific antisense riboprobe was designed for MsMob1 transcripts and a polyclonal antibody was raised against MsMob1 proteins. In situ mRNA localization as well as protein immunolocalization proved that MsMob1-like genes are specifically expressed in degenerating megaspores of normal ovules and in enlarged megaspore mother cells and embryo sacs of apomeiotic ovules. Gene products were also found in microspore tetrads at the beginning of pollen development as well as in tapetum cells of anthers undergoing programmed cell death to allow pollen dispersal at maturity. Overall results suggest that MsMob1-like genes can play a key role during the reproductive pathway in plants.

Key words

ApomeiosisgametogenesisMedicago sativa L.Mps-one-binderprogrammed cell deathreproductive mutants

Supplementary material

supp.pdf (38 kb)
Supplementary material

Copyright information

© Springer 2005

Authors and Affiliations

  • Sandra Citterio
    • 1
  • Emidio Albertini
    • 2
  • Serena Varotto
    • 3
  • Erika Feltrin
    • 3
  • Marica Soattin
    • 3
  • Gianpiero Marconi
    • 2
  • Sergio Sgorbati
    • 1
  • Margherita Lucchin
    • 3
  • Gianni Barcaccia
    • 3
  1. 1.Dipartimento di Scienze dell’Ambiente e del TerritorioUniversity of Milano – BicoccaMilanoItaly
  2. 2.Dipartimento di Biologia Vegetale e Biotecnologie AgroambientaliUniversity of PerugiaPerugiaItaly
  3. 3.Laboratory of Plant Genetics and Genomics, Dipartimento di Agronomia Ambientale e Produzioni VegetaliUniversity of Padova-AgripolisLegnaro PadovaItaly