Plant Molecular Biology

, Volume 58, Issue 5, pp 699–720

Sorghum bicolor’s Transcriptome Response to Dehydration, High Salinity and ABA

  • Christina D. Buchanan
  • Sanghyun Lim
  • Ron A. Salzman
  • Ioannis Kagiampakis
  • Daryl T. Morishige
  • Brock D. Weers
  • Robert R. Klein
  • Lee H. Pratt
  • Marie-Michèle Cordonnier-Pratt
  • Patricia E. Klein
  • John E. Mullet
Article

DOI: 10.1007/s11103-005-7876-2

Cite this article as:
Buchanan, C.D., Lim, S., Salzman, R.A. et al. Plant Mol Biol (2005) 58: 699. doi:10.1007/s11103-005-7876-2

Abstract

Genome wide changes in gene expression were monitored in the drought tolerant C4 cereal Sorghum bicolor, following exposure of seedlings to high salinity (150 mM NaCl), osmotic stress (20% polyethylene glycol) or abscisic acid (125 μM ABA). A sorghum cDNA microarray providing data on 12 982 unique gene clusters was used to examine gene expression in roots and shoots at 3- and 27-h post-treatment. Expression of ~2200 genes, including 174 genes with currently unknown functions, of which a subset appear unique to monocots and/or sorghum, was altered in response to dehydration, high salinity or ABA. The modulated sorghum genes had homology to proteins involved in regulation, growth, transport, membrane/protein turnover/repair, metabolism, dehydration protection, reactive oxygen scavenging, and plant defense. Real-time PCR was used to quantify changes in relative mRNA abundance for 333 genes that responded to ABA, NaCl or osmotic stress. Osmotic stress inducible sorghum genes identified for the first time included a beta-expansin expressed in shoots, actin depolymerization factor, inositol-3-phosphate synthase, a non-C4 NADP-malic enzyme, oleosin, and three genes homologous to 9-cis-epoxycarotenoid dioxygenase that may be involved in ABA biosynthesis. Analysis of response profiles demonstrated the existence of a complex gene regulatory network that differentially modulates gene expression in a tissue- and kinetic-specific manner in response to ABA, high salinity and water deficit. Modulation of genes involved in signal transduction, chromatin structure, transcription, translation and RNA metabolism contributes to sorghum’s overlapping but nonetheless distinct responses to ABA, high salinity, and osmotic stress. Overall, this study provides a foundation of information on sorghum’s osmotic stress responsive gene complement that will accelerate follow up biochemical, QTL and comparative studies

Keywords

ABAdehydrindroughtgene regulationmicroarraysorghum

Copyright information

© Springer 2005

Authors and Affiliations

  • Christina D. Buchanan
    • 1
  • Sanghyun Lim
    • 1
  • Ron A. Salzman
    • 1
  • Ioannis Kagiampakis
    • 2
  • Daryl T. Morishige
    • 2
  • Brock D. Weers
    • 2
  • Robert R. Klein
    • 3
  • Lee H. Pratt
    • 4
  • Marie-Michèle Cordonnier-Pratt
    • 4
  • Patricia E. Klein
    • 1
    • 5
  • John E. Mullet
    • 1
    • 2
  1. 1.Institute for Plant Genomics and BiotechnologyTexas A&M UniversityCollege StationUSA
  2. 2.Department of Biochemistry and BiophysicsTexas A&M UniversityCollege StationUSA
  3. 3.USDA-ARSSouthern Plains Agricultural Research CenterCollege StationUSA
  4. 4.Department of Plant BiologyUniversity of GeorgiaAthensUSA
  5. 5.Department of Horticultural SciencesTexas A&M UniversityCollege StationUSA