Plant Molecular Biology

, Volume 58, Issue 5, pp 699–720

Sorghum bicolor’s Transcriptome Response to Dehydration, High Salinity and ABA

Authors

  • Christina D. Buchanan
    • Institute for Plant Genomics and BiotechnologyTexas A&M University
  • Sanghyun Lim
    • Institute for Plant Genomics and BiotechnologyTexas A&M University
  • Ron A. Salzman
    • Institute for Plant Genomics and BiotechnologyTexas A&M University
  • Ioannis Kagiampakis
    • Department of Biochemistry and BiophysicsTexas A&M University
  • Daryl T. Morishige
    • Department of Biochemistry and BiophysicsTexas A&M University
  • Brock D. Weers
    • Department of Biochemistry and BiophysicsTexas A&M University
  • Robert R. Klein
    • USDA-ARSSouthern Plains Agricultural Research Center
  • Lee H. Pratt
    • Department of Plant BiologyUniversity of Georgia
  • Marie-Michèle Cordonnier-Pratt
    • Department of Plant BiologyUniversity of Georgia
  • Patricia E. Klein
    • Institute for Plant Genomics and BiotechnologyTexas A&M University
    • Department of Horticultural SciencesTexas A&M University
    • Institute for Plant Genomics and BiotechnologyTexas A&M University
    • Department of Biochemistry and BiophysicsTexas A&M University
Article

DOI: 10.1007/s11103-005-7876-2

Cite this article as:
Buchanan, C.D., Lim, S., Salzman, R.A. et al. Plant Mol Biol (2005) 58: 699. doi:10.1007/s11103-005-7876-2

Abstract

Genome wide changes in gene expression were monitored in the drought tolerant C4 cereal Sorghum bicolor, following exposure of seedlings to high salinity (150 mM NaCl), osmotic stress (20% polyethylene glycol) or abscisic acid (125 μM ABA). A sorghum cDNA microarray providing data on 12 982 unique gene clusters was used to examine gene expression in roots and shoots at 3- and 27-h post-treatment. Expression of ~2200 genes, including 174 genes with currently unknown functions, of which a subset appear unique to monocots and/or sorghum, was altered in response to dehydration, high salinity or ABA. The modulated sorghum genes had homology to proteins involved in regulation, growth, transport, membrane/protein turnover/repair, metabolism, dehydration protection, reactive oxygen scavenging, and plant defense. Real-time PCR was used to quantify changes in relative mRNA abundance for 333 genes that responded to ABA, NaCl or osmotic stress. Osmotic stress inducible sorghum genes identified for the first time included a beta-expansin expressed in shoots, actin depolymerization factor, inositol-3-phosphate synthase, a non-C4 NADP-malic enzyme, oleosin, and three genes homologous to 9-cis-epoxycarotenoid dioxygenase that may be involved in ABA biosynthesis. Analysis of response profiles demonstrated the existence of a complex gene regulatory network that differentially modulates gene expression in a tissue- and kinetic-specific manner in response to ABA, high salinity and water deficit. Modulation of genes involved in signal transduction, chromatin structure, transcription, translation and RNA metabolism contributes to sorghum’s overlapping but nonetheless distinct responses to ABA, high salinity, and osmotic stress. Overall, this study provides a foundation of information on sorghum’s osmotic stress responsive gene complement that will accelerate follow up biochemical, QTL and comparative studies

Keywords

ABAdehydrindroughtgene regulationmicroarraysorghum

Copyright information

© Springer 2005