Article

Plant Molecular Biology

, Volume 61, Issue 1, pp 123-139

A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients

  • Serge LeimanisAffiliated withUnité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix
  • , Marta HernándezAffiliated withMolecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC)
  • , Sophie FernándezAffiliated withLaboratoire de méthodologies de la détection des OGM, INRA Versailles
  • , Francine BoyerAffiliated withLaboratoire de méthodologies de la détection des OGM, INRA Versailles
  • , Malcolm BurnsAffiliated withLGC Limited
  • , Shirin BrudererAffiliated withAgency BATS
  • , Thomas GloudenAffiliated withUnité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix
  • , Neil HarrisAffiliated withLGC Limited
  • , Othmar KaeppeliAffiliated withAgency BATS
    • , Patrick PhilippAffiliated withLaboratoire Interrégional, DGCCRF
    • , Maria PlaAffiliated withMolecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC)
    • , Pere PuigdomènechAffiliated withMolecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC)
    • , Marc VaitilingomAffiliated withTEPRAL—Brasseries KRONENBOURG
    • , Yves BertheauAffiliated withLaboratoire de méthodologies de la détection des OGM, INRA Versailles
    • , José RemacleAffiliated withUnité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix Email author 

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Abstract

A multiplex DNA microarray chip was developed for simultaneous identification of nine genetically modified organisms (GMOs), five plant species and three GMO screening elements, i.e. the 35S promoter, the nos terminator and the nptII gene. The chips also include several controls, such as that for the possible presence of CaMV. The on-chip detection was performed directly with PCR amplified products. Particular emphasis was placed on the reduction of the number of PCR reactions required and on the number of primers present per amplification tube. The targets were biotin labelled and the arrays were detected using a colorimetric methodology. Specificity was provided by specific capture probes designed for each GMO and for the common screening elements. The sensitivity of the assay was tested by experiments carried out in five different laboratories. The limit of detection was lower than 0.3% GMO for all tests and in general around 0.1% for most GMOs. The chip detection system complies with the requirements of current EU regulations and other countries where thresholds are established for the labelling of GMO.

Keywords

colorimetric detection GMO GMOchips microarray