Plant Molecular Biology

, Volume 61, Issue 1, pp 123–139

A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients

  • Serge Leimanis
  • Marta Hernández
  • Sophie Fernández
  • Francine Boyer
  • Malcolm Burns
  • Shirin Bruderer
  • Thomas Glouden
  • Neil Harris
  • Othmar Kaeppeli
  • Patrick Philipp
  • Maria Pla
  • Pere Puigdomènech
  • Marc Vaitilingom
  • Yves Bertheau
  • José Remacle
Article

DOI: 10.1007/s11103-005-6173-4

Cite this article as:
Leimanis, S., Hernández, M., Fernández, S. et al. Plant Mol Biol (2006) 61: 123. doi:10.1007/s11103-005-6173-4

Abstract

A multiplex DNA microarray chip was developed for simultaneous identification of nine genetically modified organisms (GMOs), five plant species and three GMO screening elements, i.e. the 35S promoter, the nos terminator and the nptII gene. The chips also include several controls, such as that for the possible presence of CaMV. The on-chip detection was performed directly with PCR amplified products. Particular emphasis was placed on the reduction of the number of PCR reactions required and on the number of primers present per amplification tube. The targets were biotin labelled and the arrays were detected using a colorimetric methodology. Specificity was provided by specific capture probes designed for each GMO and for the common screening elements. The sensitivity of the assay was tested by experiments carried out in five different laboratories. The limit of detection was lower than 0.3% GMO for all tests and in general around 0.1% for most GMOs. The chip detection system complies with the requirements of current EU regulations and other countries where thresholds are established for the labelling of GMO.

Keywords

colorimetricdetectionGMOGMOchipsmicroarray

Abbreviations

CaMV

cauliflower mosaic virus

CTAB

hexadecyltrimethylammonium bromide

DNA

deoxyribonucleic acid

EC

European Commission

EU

European Union

GMO

genetically modified organism

NptII

neomycin phospho transferase originating from Streptomyces hygroscopicus

P-35S

promoter 35S originating from cauliflower mosaic virus

Pat

phosphinotricin-N-acetyl transferase

PCR

polymerase chain reaction

RRS

roundup ready® soybean

T35S

terminator 35S originating from cauliflower mosaic virus

Ta

annealing temperature

T-nos

terminator nopaline synthase originating from Agrobacterium tumefaciens

UNG

uracil-N-glycosylase

Copyright information

© Springer 2006

Authors and Affiliations

  • Serge Leimanis
    • 1
  • Marta Hernández
    • 2
  • Sophie Fernández
    • 3
  • Francine Boyer
    • 3
  • Malcolm Burns
    • 4
  • Shirin Bruderer
    • 5
  • Thomas Glouden
    • 1
  • Neil Harris
    • 4
  • Othmar Kaeppeli
    • 5
  • Patrick Philipp
    • 6
  • Maria Pla
    • 2
  • Pere Puigdomènech
    • 2
  • Marc Vaitilingom
    • 7
  • Yves Bertheau
    • 3
  • José Remacle
    • 1
  1. 1.Unité de Recherche en Biologie Cellulaire (URBC)Faculté Universitaire Notre Dame de la PaixNamurBelgium
  2. 2.Molecular Genetics DepartmentInstituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC)BarcelonaSpain
  3. 3.Laboratoire de méthodologies de la détection des OGMINRA VersaillesVersailles CedexFrance
  4. 4.LGC LimitedTeddington, MiddlesexUK
  5. 5.Agency BATSBaseSwitzerland
  6. 6.Laboratoire InterrégionalDGCCRFIllkirch-GraffenstadenFrance
  7. 7.TEPRAL—Brasseries KRONENBOURGStrasbourgFrance