A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients
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A multiplex DNA microarray chip was developed for simultaneous identification of nine genetically modified organisms (GMOs), five plant species and three GMO screening elements, i.e. the 35S promoter, the nos terminator and the nptII gene. The chips also include several controls, such as that for the possible presence of CaMV. The on-chip detection was performed directly with PCR amplified products. Particular emphasis was placed on the reduction of the number of PCR reactions required and on the number of primers present per amplification tube. The targets were biotin labelled and the arrays were detected using a colorimetric methodology. Specificity was provided by specific capture probes designed for each GMO and for the common screening elements. The sensitivity of the assay was tested by experiments carried out in five different laboratories. The limit of detection was lower than 0.3% GMO for all tests and in general around 0.1% for most GMOs. The chip detection system complies with the requirements of current EU regulations and other countries where thresholds are established for the labelling of GMO.
- Ahmed, F.E. (2002) Detection of genetically modified organisms in foods. Trends Biotechnol. 20: pp. 215-223 CrossRef
- Alexandre, I., Hamels, S., Dufour, S., Mollet, J., Zammatteo, N., Longueville, F., Gala, J.L., Remacle, J. (2001) Colorimetric silver detection of DNA microarrays. Anal. Biochem. 295: pp. 1-8 CrossRef
- Bordoni, R., Germini, A., Mezzelani, A., Marchelli, R., Bellis, G. (2005) A microarray platform for parallel detection of five transgenic events in foods: a combined polymerase chain reaction-ligation detection reaction-universal array method. J. Agric. Food Chem. 53: pp. 912-918 CrossRef
- Bordoni, R., Mezzelani, A., Consolandi, C., Frosini, A., Rizzi, E., Castiglioni, B., Salati, C., Marmiroli, N., Marchelli, R., Rossi Bernardi, L., Battaglia, C., Bellis, G. (2004) Detection and quantitation of genetically modified maize (Bt-176 transgenic maize) by applying ligation detection reaction and universal array technology. J. Agric. Food Chem. 52: pp. 1049-1054
- Dellaporta, S.L., Wood, J., Hicks, J.B. (1983) A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1: pp. 19-21
- Fernández, S., Charles-Delobel, C., Geldreich, A., Berthier, G., Boyer, F., Collonnier, C., Coué-Philippe, G., Duplan, M.N., Kebdani, N., Romaniuk, M., Bertheau, Y. (2005) Quantification of the 35S promoter in DNA extracts from genetically modified organisms using real time PCR Part I: operating procedure. J. AOAC Int. 88: pp. 547-557
- Garcia-Canas, V., Gonzalez, R., Cifuentes, A. (2004) Sensitive and simultaneous analysis of five transgenic maizes using multiplex polymerase chain reaction, capillary gel electrophoresis, and laser-induced fluorescence. Electrophoresis 25: pp. 2219-2226 CrossRef
- Germini, A., Rossi, S., Zanetti, A., Corradini, R., Fogher, C., Marchelli, R (2005) Development of a peptide nucleic acid array platform for the detection of genetically modified organisms in food. J. Agric. Food Chem. 53: pp. 3958-3962 CrossRef
- Germini, A., Zanetti, A., Salati, C., Rossi, S., Forre, C., Schmid, S., Marchelli, R., Fogher, C. (2004) Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. J. Agric. Food Chem. 52: pp. 3275-3280 CrossRef
- Hamels, S., Gala, J.-L., Dufour, S., Vannuffel, P., Zammatteo, N., Remacle, J. (2001) Consensus PCR and microarray for diagnosis of the genus Staphylococcus, species, and methicillin resistance. Biotechniques 31: pp. 1364-1366
- Hemmer, W. 1997. Foods derived from genetically modified organisms and detection methods. Biosafety Research and Assessment of Technology Impacts of the Swiss Priority Programme Biotechnology of the Swiss National Science Foundation, Report 2/97, Basel, Switzerland, pp. 14–20.
- Hernández, M., Duplan, M.-N., Berthier, G., Vaitilingom, M., Hauser, W., Freyer, R., Pla, M., Bertheau, Y. (2004) Development and comparison of four real-time polymerase chain reaction systems for specific detection and quantification of Zea mays L. J. Agric. Food Chem. 52: pp. 4632-4637 CrossRef
- Hernández, M., Rodriguez-Lazaro, D., Zhang, D., Esteve, T., Pla, M., Prat, S. (2005) Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize lines: Bt11, MON810, T25, and GA21. J. Agric. Food Chem. 53: pp. 3333-3337 CrossRef
- James C. 2004. Preview: Global Status of Commercialized Biotech/GM Crops: 2004. ISAAA Briefs. No. 32, Ithaca, NY, Jan 2004, p. 12.
- Lee, M.L., Kuo, F.C., Whitmore, G.A., Sklar, J. (2000) Importance of replication in microarray gene expression studies: statistical methods and evidence from repetitive cDNA hybridizations. Proc. Natl. Acad. Sci. USA. 97: pp. 9834-9839 CrossRef
- Lipp, M., Anklam, E., Stave, J.W., Lipp, M., Anklam, E., Stave, J.W. (2000) Validation of an immunoassay for detection and quantitation of a genetically modified soybean in food and food fractions using reference materials: interlaboratory study. J. AOAC Int. 83: pp. 919-927
- Matsuoka, T., Kuribara, H., Akiyama, H., Miura, H., Goda, Y., Kusukabe, Y., Isshiki, K., Toyoda, M., Hino, A. (2001) A multiplex PCR method of detecting recombinant DNAs from five lines of genetically modified maize. J. Food Hyg. Soc. Jpn. 42: pp. 28-32
- Padgette, S.R., Kolacz, K.H., Delannay, X., Re, D.B., La Vallee, B.J., Tinius, C.N., Rhodes, W.K., Otero, Y.I., Barry, G.F., Eichholz, D.A., Peschke, V.M., Nida, D.L., Taylor, N.B., Kishore, G.M. (1995) Development, identification and characterization of a glyphosate-tolerant soybean line. Crop Sci. 35: pp. 1451-1461 CrossRef
- Permingeat, H.R., Reggiardo, M.I., Vallejos, R.H. (2002) Detection and quantification of transgenes in grains by multiplex and real-time PCR. J. Agric. Food Chem. 50: pp. 4431-4436 CrossRef
- Regulation (EC) No 258/97 of the European Parliament and the Council of 27 January 1997 concerning novel foods and novel food ingredients. 1997. Official Journal of the European Communities. No L 43, pp. 1–7.
- Regulation (EC) No 18/2001 of the European Parliament and of the Council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90/220/EEC – commission declaration. 2001. Official Journal of the European Communities. No L 106, pp. 1–39.
- Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. 2003. Official Journal of the European Communities, No L 268, pp. 1–23.
- Regulation (EC) No 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending directive 2001/18/EC. 2003. Official Journal of the European Communities. No L 268, pp. 24–28.
- Rogers, S.O., Bendich, A.J. (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5: pp. 69-76 CrossRef
- Rønning, S.B., Vaïtilingom, M., Berdal, K.G., Holst-Jensen, A. (2003) Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays). Eur. Res. Food Technol. 216: pp. 347-354
- Rudi, K., Rud, I., Holck, A. (2003) A novel multiplex quantitative DNA array based PCR (MQDA-PCR) for quantification of transgenic maize in food and feed. Nucleic Acids Res. 31: pp. e62 CrossRef
- Stave, J.W. (2002) Protein immunoassay methods for detection of biotech crops: applications, limitations, and practical considerations. J. AOAC Int. 85: pp. 780-786
- Terry, C.F., Harris, N. (2001) Event-specific detection of Roundup Ready soya using two different real time PCR detection chemistries. Eur. Food Res. Technol. 213: pp. 425-431 CrossRef
- Windels, P., Bertrand, S., Depicker, A., Moens, W., Bockstaele, E., Loose, M. (2003) Qualitative and event-specific PCR Real-time detection methods for StarLink maize. Eur. Food Res. Technol. 216: pp. 259-263
- Zammatteo, N., Jeanmart, L., Hamels, S., Courtois, S., Louette, P., Hevesi, L., Remacle, J. (2000) Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarrays. Anal. Biochem. 280: pp. 143-150 CrossRef
- Zimmermann, A., Hemmer, W., Liniger, M., Lüthy, J., Pauli, U. (1998) A sensitive detection method for genetically modified MaisGard corn using a nested PCR-system. Lebensm.-Wiss. -Technol. 31: pp. 664-667 CrossRef
- A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients
Plant Molecular Biology
Volume 61, Issue 1-2 , pp 123-139
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- 1. Unité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix, 61, rue de Bruxelles, Namur, 5000, Belgium
- 2. Molecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC), Jordi Girona Salgado 18-26, Barcelona, 08034, Spain
- 3. Laboratoire de méthodologies de la détection des OGM, INRA Versailles, Route de Saint Cyr RD 10, Versailles Cedex, 78026, France
- 4. LGC Limited, Queens Road, Teddington, Middlesex, TW11 OLY, UK
- 5. Agency BATS, Clarastrasse 13, Base, 4058, Switzerland
- 6. Laboratoire Interrégional, DGCCRF, 67 Chemin du routoir, Illkirch-Graffenstaden, 67400, France
- 7. TEPRAL—Brasseries KRONENBOURG, 68, route d’Oberhausbergen, Strasbourg, 67037, France