A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients
Received: 16 July 2005 Accepted: 23 December 2005 DOI:
Cite this article as: Leimanis, S., Hernández, M., Fernández, S. et al. Plant Mol Biol (2006) 61: 123. doi:10.1007/s11103-005-6173-4 Abstract
A multiplex DNA microarray chip was developed for simultaneous identification of nine genetically modified organisms (GMOs), five plant species and three GMO screening elements, i.e. the
35S promoter, the nos terminator and the nptII gene. The chips also include several controls, such as that for the possible presence of CaMV. The on-chip detection was performed directly with PCR amplified products. Particular emphasis was placed on the reduction of the number of PCR reactions required and on the number of primers present per amplification tube. The targets were biotin labelled and the arrays were detected using a colorimetric methodology. Specificity was provided by specific capture probes designed for each GMO and for the common screening elements. The sensitivity of the assay was tested by experiments carried out in five different laboratories. The limit of detection was lower than 0.3% GMO for all tests and in general around 0.1% for most GMOs. The chip detection system complies with the requirements of current EU regulations and other countries where thresholds are established for the labelling of GMO. Keywords colorimetric detection GMO GMOchips microarray Abbreviations CaMV
cauliflower mosaic virus
genetically modified organism
neomycin phospho transferase originating from
Streptomyces hygroscopicus P-35S
promoter 35S originating from cauliflower mosaic virus
polymerase chain reaction
roundup ready® soybean
terminator 35S originating from cauliflower mosaic virus
terminator nopaline synthase originating from
Agrobacterium tumefaciens UNG
Serge Leimanis, Marta Hernández, Sophie Fernández: These authors contributed equally to this paper.
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