Plant Molecular Biology

, Volume 58, Issue 3, pp 367–384

Identification of differentially expressed genes in leaf and root between wheat hybrid and its parental inbreds using PCR-based cDNA subtraction

  • Yingyin Yao
  • Zhongfu Ni
  • Yinhong Zhang
  • Yan Chen
  • Yuhua Ding
  • Zongfu Han
  • Zhiyong Liu
  • Qixin Sun
Article

DOI: 10.1007/s11103-005-5102-x

Cite this article as:
Yao, Y., Ni, Z., Zhang, Y. et al. Plant Mol Biol (2005) 58: 367. doi:10.1007/s11103-005-5102-x

Abstract

Heterosis was defined as the advantage of hybrid performance over its parents in terms of growth and productivity. Previous studies showed that differential gene expression between hybrids and their parents is responsible for the heterosis; however, information on systematic identification and characterization of the differentially expressed genes are limited. In this study, an interspecific hybrid between common wheat (Triticum aestivum. L., 2n = 6x = 42, AABBDD) line 3338 and spelt (Triticum spelta L. 2n = 6x = 42, AABBDD) line 2463 was found to be highly heterotic in both aerial growth and root related traits, and was then used for expression assay. A modified suppression subtractive hybridization (SSH) was used to generate four subtracted cDNA libraries, and 748 nonreduandant cDNAs were obtained, among which 465 had high sequence similarity to the GenBank entries and represent diverse of functional categories, such as metabolism, cell growth and maintenance, signal transduction, photosynthesis, response to stress, transcription regulation and others. The expression patterns of 68.2% SSH-derived cDNAs were confirmed by reverse Northern blot, and semi-quantitative RT-PCR exhibited the similar results (72.2%). And it was concluded that the genes differentially expressed between hybrids and their parents involved in diverse physiological process pathway, which might be responsible for the observed heterosis.

Keywords

gene expressionheterosishybrid wheatsuppression subtractive hybridization

Abbreviations

BPH

best-parent heterosis

GO

gene ontology

LRL

longest root length

MPH

mid-parent heterosis

RAD

root average diameter

RDW

root dry weight

RSA

root surface area

RSR

root/shoot ratio

RTN

root tips number

RT-PCR

reverse transcription polymerase chain reaction

RV

root volume

SSH

suppression subtractive hybridization

TL

taproot length

TN

taproot number

TRL

total root length

Supplementary material

supp.pdf (202 kb)
Supplementary material

Copyright information

© Springer 2005

Authors and Affiliations

  • Yingyin Yao
    • 1
  • Zhongfu Ni
    • 1
  • Yinhong Zhang
    • 1
  • Yan Chen
    • 1
  • Yuhua Ding
    • 1
  • Zongfu Han
    • 1
  • Zhiyong Liu
    • 1
  • Qixin Sun
    • 1
    • 2
  1. 1.Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic ImprovementMinistry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityBeijingChina
  2. 2.Department of Plant Genetics & BreedingChina Agricultural University BeijingChina