Plant Molecular Biology

, Volume 60, Issue 2, pp 185–199

Comparative Structural and Functional Characterization of Sorghum and Maize Duplications Containing Orthologous Myb Transcription Regulators of 3-Deoxyflavonoid Biosynthesis

Authors

  • Jayanand Boddu
    • Department of Crop & Soil SciencesPennsylvania State University
    • Department of Agronomy and Plant GeneticsUniversity of Minnesota
  • Cizhong Jiang
    • Department of Genetics, Development and Cell BiologyIowa State University
    • Cold Spring Harbor Laboratory
  • Vineet Sangar
    • Department of Crop & Soil SciencesPennsylvania State University
  • Terry Olson
    • Department of Genetics, Development and Cell BiologyIowa State University
  • Thomas Peterson
    • Department of Genetics, Development and Cell BiologyIowa State University
    • Department of Crop & Soil SciencesPennsylvania State University
Article

DOI: 10.1007/s11103-005-3568-1

Cite this article as:
Boddu, J., Jiang, C., Sangar, V. et al. Plant Mol Biol (2006) 60: 185. doi:10.1007/s11103-005-3568-1

Abstract

Sequence characterization of the genomic region of sorghum yellow seed1 shows the presence of two genes that are arranged in a head to tail orientation. The two duplicated gene copies, y1 and y2 are separated by a 9.084 kbp intergenic region, which is largely composed of highly repetitive sequences. The y1 is the functional copy, while the y2 may represent a pseudogene; there are several sequence indels and rearrangements within the putative coding region of y2. The y1 gene encodes a R2R3 type of Myb domain protein that regulates the expression of chalcone synthase, chalcone isomerase and dihydroflavonol reductase genes required for the biosynthesis of 3-deoxyflavonoids. Expression of y1 can be observed throughout the plant and it represents a combination of expression patterns produced by different alleles of the maize p1. Comparative sequence analysis within the coding regions and flanking sequences of y1, y2 and their maize and teosinte orthologs show local rearrangements and insertions that may have created modified regulatory regions. These micro-colinearity modifications possibly are responsible for differential patterns of expression in maize and sorghum floral and vegetative tissues. Phylogenetic analysis indicates that sorghum y1 and y2 sequences may have arisen by gene duplication mechanisms and represent an evolutionarily parallel event to the duplication of maize p2 and p1 genes.

Keywords

3-deoxyflavonoidsanthocyaninsflavonoidgene structuresorghummaizeMybpericarpphlobaphenesphytoalexins

Copyright information

© Springer 2006