Plant Molecular Biology

, Volume 57, Issue 6, pp 819–833

Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells

Authors

  • Keishi Osakabe
    • Department of Plant BiotechnologyNational Institute of Agrobiological Sciences
  • Kiyomi Abe
    • Department of Plant BiotechnologyNational Institute of Agrobiological Sciences
  • Hiroaki Yamanouchi
    • Institute of Radiation BreedingNational Institute of Agrobiological Sciences
  • Toshio Takyuu
    • Institute of Radiation BreedingNational Institute of Agrobiological Sciences
  • Terutaka Yoshioka
    • Institute of Radiation BreedingNational Institute of Agrobiological Sciences
  • Yuji Ito
    • Institute of Radiation BreedingNational Institute of Agrobiological Sciences
  • Tomohiko Kato
    • Kazusa DNA Research Institute
  • Satoshi Tabata
    • Kazusa DNA Research Institute
  • Shunsuke Kurei
    • Division of Biological Science, Graduate School of ScienceNagoya University
  • Yasushi Yoshioka
    • Division of Biological Science, Graduate School of ScienceNagoya University
  • Yasunori Machida
    • Division of Biological Science, Graduate School of ScienceNagoya University
  • Motoaki Seki
    • RIKEN Genomic Sciences Center (GSC)RIKEN Yokohama Institute
  • Masatomo Kobayashi
    • Experimental Plant DivisionRIKEN BioResource Center
  • Kazuo Shinozaki
    • RIKEN Genomic Sciences Center (GSC)RIKEN Yokohama Institute
  • Hiroaki Ichikawa
    • Department of Plant BiotechnologyNational Institute of Agrobiological Sciences
    • Department of Plant BiotechnologyNational Institute of Agrobiological Sciences
Article

DOI: 10.1007/s11103-005-2187-1

Cite this article as:
Osakabe, K., Abe, K., Yamanouchi, H. et al. Plant Mol Biol (2005) 57: 819. doi:10.1007/s11103-005-2187-1

Abstract

Rad51 paralogs belong to the Rad52 epistasis group of proteins and are involved in homologous recombination (HR), especially the assembly and stabilization of Rad51, which is a homolog of RecA in eukaryotes. We previously cloned and characterized two RAD51 paralogous genes in Arabidopsis, named AtRAD51C and AtXRCC3, which are considered the counterparts of human RAD51C and XRCC3, respectively. Here we describe the identification of RAD51B homologue in Arabidopsis, AtRAD51B. We found a higher expression of AtRAD51B in flower buds and roots. Expression of AtRAD51B was induced by genotoxic stresses such as ionizing irradiation and treatment with a cross-linking reagent, cisplatin. Yeast two-hybrid analysis showed that AtRad51B interacted with AtRad51C. We also found and characterized T-DNA insertion mutant lines. The mutant lines were devoid of AtRAD51B expression, viable and fertile. The mutants were moderately sensitive to γ-ray and hypersensitive to cisplatin. Our results suggest that AtRAD51B gene product is involved in the repair of double-strand DNA breaks (DSBs) via HR

Keywords

Arabidopsiscisplatinγ-rayhomologous recombinationRad51 paralogsT-DNA tagging

Abbreviations

RACE

rapid amplification of cDNA ends

RT-PCR

reverse transcriptase polymerase chain reaction

X-gal

5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside

Copyright information

© Springer 2005