Article

Plant Molecular Biology

, Volume 57, Issue 6, pp 819-833

First online:

Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells

  • Keishi OsakabeAffiliated withDepartment of Plant Biotechnology, National Institute of Agrobiological Sciences
  • , Kiyomi AbeAffiliated withDepartment of Plant Biotechnology, National Institute of Agrobiological Sciences
  • , Hiroaki YamanouchiAffiliated withInstitute of Radiation Breeding, National Institute of Agrobiological Sciences
  • , Toshio TakyuuAffiliated withInstitute of Radiation Breeding, National Institute of Agrobiological Sciences
  • , Terutaka YoshiokaAffiliated withInstitute of Radiation Breeding, National Institute of Agrobiological Sciences
  • , Yuji ItoAffiliated withInstitute of Radiation Breeding, National Institute of Agrobiological Sciences
  • , Tomohiko KatoAffiliated withKazusa DNA Research Institute
  • , Satoshi TabataAffiliated withKazusa DNA Research Institute
  • , Shunsuke KureiAffiliated withDivision of Biological Science, Graduate School of Science, Nagoya University
    • , Yasushi YoshiokaAffiliated withDivision of Biological Science, Graduate School of Science, Nagoya University
    • , Yasunori MachidaAffiliated withDivision of Biological Science, Graduate School of Science, Nagoya University
    • , Motoaki SekiAffiliated withRIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute
    • , Masatomo KobayashiAffiliated withExperimental Plant Division, RIKEN BioResource Center
    • , Kazuo ShinozakiAffiliated withRIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute
    • , Hiroaki IchikawaAffiliated withDepartment of Plant Biotechnology, National Institute of Agrobiological Sciences
    • , Seiichi TokiAffiliated withDepartment of Plant Biotechnology, National Institute of Agrobiological Sciences Email author 

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Abstract

Rad51 paralogs belong to the Rad52 epistasis group of proteins and are involved in homologous recombination (HR), especially the assembly and stabilization of Rad51, which is a homolog of RecA in eukaryotes. We previously cloned and characterized two RAD51 paralogous genes in Arabidopsis, named AtRAD51C and AtXRCC3, which are considered the counterparts of human RAD51C and XRCC3, respectively. Here we describe the identification of RAD51B homologue in Arabidopsis, AtRAD51B. We found a higher expression of AtRAD51B in flower buds and roots. Expression of AtRAD51B was induced by genotoxic stresses such as ionizing irradiation and treatment with a cross-linking reagent, cisplatin. Yeast two-hybrid analysis showed that AtRad51B interacted with AtRad51C. We also found and characterized T-DNA insertion mutant lines. The mutant lines were devoid of AtRAD51B expression, viable and fertile. The mutants were moderately sensitive to γ-ray and hypersensitive to cisplatin. Our results suggest that AtRAD51B gene product is involved in the repair of double-strand DNA breaks (DSBs) via HR

Keywords

Arabidopsis cisplatin γ-ray homologous recombination Rad51 paralogs T-DNA tagging