Plant Molecular Biology

, Volume 59, Issue 6, pp 909–925

Dynamic Histone Acetylation of Late Embryonic Genes during Seed Germination

Authors

    • Canadian Forest ServiceNatural Resources Canada
  • George C. C. Tai
    • Potato Research CentreAgriculture and Agri-Food Canada
  • Tannis Beardmore
    • Canadian Forest ServiceNatural Resources Canada
Article

DOI: 10.1007/s11103-005-2081-x

Cite this article as:
Tai, H.H., Tai, G.C.C. & Beardmore, T. Plant Mol Biol (2005) 59: 909. doi:10.1007/s11103-005-2081-x

Abstract

Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis – RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at 1 day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes.

Keywords

chromatingene expressionhistone acetylationhistone deacetylasemicroarrayseed germination

Abbreviations

ABRE

abscisic acid response element

ANOVA

analysis of variance

ChIP

chromatin immunoprecipitation

DAI

days after imbibition

DNA

deoxyribonucleic acid

HAT

histone acetyltransferase

HDAC(s)

histone deacetylase(s)

LIMMA

linear models for microarray analysis

MS

Murashige and Skoog

qPCR

quantitative PCR

RNA

ribonucleic acid

RTqPCR

reverse transcription quantitative PCR

TSA

Trichostatin A

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© Springer 2005