Plant Molecular Biology

, Volume 59, Issue 4, pp 619–630

Conservation and Diversification of SCARECROW in Maize

  • Jun Lim
  • Jee W. Jung
  • Chae Eun Lim
  • Mi-Hyun Lee
  • Bong Jun Kim
  • Miran Kim
  • Wesley B. Bruce
  • Philip N. Benfey
Article

DOI: 10.1007/s11103-005-0578-y

Cite this article as:
Lim, J., Jung, J.W., Lim, C.E. et al. Plant Mol Biol (2005) 59: 619. doi:10.1007/s11103-005-0578-y
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Abstract

The SCARECROW (SCR) gene in Arabidopsis is required for asymmetric cell divisions responsible for ground tissue formation in the root and shoot. Previously, we reported that Zea mays SCARECROW (ZmSCR) is the likely maize ortholog of SCR. Here we describe conserved and divergent aspects of ZmSCR. Its ability to complement the Arabidopsisscr mutant phenotype suggests conservation of function, yet its expression pattern during embryogenesis and in the shoot system indicates divergence. ZmSCR expression was detected early during embryogenesis and localized to the endodermal lineage in the root, showing a gradual regionalization of expression. Expression of ZmSCR appeared to be analogous to that of SCR during leaf formation. However, its absence from the maize shoot meristem and its early expression pattern during embryogenesis suggest a diversification of ZmSCR in the patterning processes in maize. To further investigate the evolutionary relationship of SCR and ZmSCR, we performed a phylogenetic analysis using Arabidopsis, rice and maize SCARECROW-LIKE genes (SCLs). We found SCL23 to be the most closely related to SCR in both eudicots and monocots, suggesting that a gene duplication resulting in SCR and SCL23 predates the divergence of dicots and monocots.

Keywords

Arabidopsis GRAS family pattern formation root development SCARECROW 

Supplementary material

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Supplementary material

Copyright information

© Springer 2005

Authors and Affiliations

  • Jun Lim
    • 1
    • 2
    • 3
  • Jee W. Jung
    • 4
  • Chae Eun Lim
    • 2
  • Mi-Hyun Lee
    • 2
    • 3
  • Bong Jun Kim
    • 2
    • 3
  • Miran Kim
    • 2
    • 3
  • Wesley B. Bruce
    • 5
  • Philip N. Benfey
    • 4
  1. 1.Department of Molecular BiotechnologyKonkuk UniversitySeoulKorea
  2. 2.Bio/Molecular Informatics Center (BMIC)Konkuk UniversitySeoulKorea
  3. 3.Department of Advanced Technology FusionKonkuk UniversitySeoulKorea
  4. 4.Department of BiologyDuke UniversityDurhamUSA
  5. 5.Pioneer Hi-Bred International Inc.JohnstonUSA

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