Plant Molecular Biology

, Volume 59, Issue 3, pp 469–484

Whole Gene Family Expression and Drought Stress Regulation of Aquaporins

Authors

    • Department of Plant BiochemistryLund University
  • Laure Fraysse
    • Department of Plant BiochemistryLund University
  • Sara Sjövall-Larsen
    • Department of Plant BiochemistryLund University
  • Sofia Gustavsson
    • Department of Plant BiochemistryLund University
  • Maria Fellert
    • Department of Plant BiochemistryLund University
  • Maria Karlsson
    • Department of Plant BiochemistryLund University
  • Urban Johanson
    • Department of Plant BiochemistryLund University
  • Per Kjellbom
    • Department of Plant BiochemistryLund University
Article

DOI: 10.1007/s11103-005-0352-1

Cite this article as:
Alexandersson, E., Fraysse, L., Sjövall-Larsen, S. et al. Plant Mol Biol (2005) 59: 469. doi:10.1007/s11103-005-0352-1

Abstract

Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level.

Key words

aquaporinsArabidopsisdrought stressmicroarrayMIPQ-RT-PCR

Abbreviations

ABA

abscisic acid

AQP

aquaporin

MIP

major intrinsic protein

NIP

NOD26-like intrinsic protein

PIP

plasma membrane intrinsic protein

Q-RT-PCR

quantitative real-time reverse transcriptase PCR

SIP

small basic intrinsic protein

TIP

tonoplast intrinsic protein

UTR

un-translated region

Supplementary material

supp.pdf (186 kb)
Supplementary material (36 KB)

Copyright information

© Springer 2005