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Systemic response to aphid infestation by Myzus persicae in the phloem of Apium graveolens

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Abstract

Little is known about the molecular processes involved in the phloem response to aphid feeding. We investigated molecular responses to aphid feeding on celery (Apium graveolenscv. Dulce) plants infested with the aphid Myzus persicae, as a means of identifying changes in phloem function. We used celery as our model species as it is easy to separate the phloem from the surrounding tissues in the petioles of mature leaves of this species. We generated a total of 1187 expressed sequence tags (ESTs), corresponding to 891 non-redundant genes. We analysed these ESTs in silico after cDNA macroarray hybridisation. Aphid feeding led to significant increase in RNA accumulation for 126 different genes. Different patterns of deregulation were observed, including transitory or stable induction 3 or 7 days after infestation. The genes affected belonged to various functional categories and were induced systemically in the phloem after infestation. In particular, genes involved in cell wall modification, water transport, vitamin biosynthesis, photosynthesis, carbon assimilation and nitrogen and carbon mobilisation were up-regulated in the phloem. Further analysis of the response in the phloem or xylem suggested that a component of the response was developed more specifically in the phloem. However, this component was different from the stress responses in the phloem driven by pathogen infection. Our results indicate that the phloem is actively involved in multiple adjustments, recruiting metabolic pathways and in structural changes far from aphid feeding sites. However, they also suggest that the phloem displays specific mechanisms that may not be induced in other tissues.

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Abbreviations

CMV:

Cucumber mosaic cucumovirus

EST:

Expressed sequence tag

JA:

Jasmonic acid

PR:

Pathogenesis-related

SA:

Salicylic acid

References

  • K. Akashi N. Nishimura Y. Ishida A. Yokota (2004) ArticleTitlePotent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon Biochem Biophys Res Commun 323 72–78

    Google Scholar 

  • C.R. Allagulova F.R. Gimalov F.M. Shakirova V.A. Vakhitov (2003) ArticleTitleThe plant dehydrins: structure and putative functions Biochemistry (Mosc) 68 945–951

    Google Scholar 

  • M.S. Almeida K.M. Cabral R.B. Zingali E. Kurtenbach (2000) ArticleTitleCharacterization of two novel defense peptides from pea (Pisum sativum) seeds Arch Biochem Biophys 378 278–286

    Google Scholar 

  • S.F. Altschul T.L. Madden A.A. Schäffer J. Zhang Z. Zhang W. Miller D.J. Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acid Res 25 3389–3402 Occurrence Handle10.1093/nar/25.17.3389

    Article  Google Scholar 

  • V.H. Argandona M. Chaman L. Cardemil O. Munoz G.E. Zuniga L.J. Corcuera (2001) ArticleTitleEthylene production and peroxidase activity in aphid-infested barley J Chem Ecol 27 53–68 Occurrence Handle10.1023/A:1005615932694 Occurrence Handle1:CAS:528:DC%2BD3MXisFKrtb4%3D Occurrence Handle11382067

    Article  CAS  PubMed  Google Scholar 

  • T. Asano T. Masumura H. Kusano S. Kikuchi A. Kurita H. Shimada K. Kadowaki (2002) ArticleTitleConstruction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem Plant J 32 401–408 Occurrence Handle10.1046/j.1365-313X.2002.01423.x Occurrence Handle1:CAS:528:DC%2BD38Xpt12msL8%3D Occurrence Handle12410817

    Article  CAS  PubMed  Google Scholar 

  • I.T. Baldwin R. Halitschke A. Kessler U. Schittko (2001) ArticleTitleMerging molecular and ecological approaches in plant-insect interactions Curr Opin Plant Biol 4 351–358

    Google Scholar 

  • F. Barrieu F. Chaumont M.J. Chrispeels (1998) ArticleTitleHigh expression of the tonoplast aquaporin ZmTIP1 in epidermal and conducting tissues of maize Plant Physiol 117 1153–1163

    Google Scholar 

  • F.C. Belanger T. Leustek B. Chu A.L. Kriz (1995) ArticleTitleEvidence for the thiamine biosynthetic pathway in higher-plant plastids and its developmental regulation Plant Mol Biol 29 809–821

    Google Scholar 

  • A. Bergman M.L. Siegal (2003) ArticleTitleEvolutionary capacitance as a general feature of complex gene networks Nature 424 549–552

    Google Scholar 

  • M. Berrocal-Lobo A. Segura M. Moreno G. Lopez F. Garcia-Olmedo A. Molina (2002) ArticleTitleSnakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection Plant Physiol 128 951–961

    Google Scholar 

  • K. Birnbaum D.E. Shasha J.Y. Wang J.W. Jung G.M. Lambert D.W. Galbraith P.N. Benfey (2003) ArticleTitleA gene expression map of the Arabidopsisroot Science 12 IssueID302 1956–196

    Google Scholar 

  • O. Blokhina E. Virolainen K.V. Fagerstedt (2003) ArticleTitleAntioxidants, oxidative damage and oxygen deprivation stress: a review Ann Bot (Lond) 91 179–194

    Google Scholar 

  • C. Breda C. Sallaud J. el-Turk D. Buffard I. Kozak Particlede R. Esnault A. Kondorosi (1996) ArticleTitleDefense reaction in Medicago sativa: a gene encoding a class 10 PR protein is expressed in vascular bundles Mol. Plant Microbe Interact 9 713–719

    Google Scholar 

  • H. Breiteneder K. Hoffmann-Sommergruber O’Riordain G. M. Susani H. Ahorn C. Ebner Kraft D. Scheiner O. (1995) ArticleTitleMolecular characterization of Api g 1, the major allergen of celery (Apium graveolens), and its immunological and structural relationships to a group of 17-kDa tree pollen allergens Eur J Biochem 233 484–489 Occurrence Handle10.1111/j.1432-1033.1995.484_2.x Occurrence Handle1:CAS:528:DyaK2MXptlKru70%3D Occurrence Handle7588792

    Article  CAS  PubMed  Google Scholar 

  • A. Cherqui WF. Tjallingii (2000) ArticleTitleSalivary proteins of aphids, a pilot study on identification, separation and immunolocalisation J Insect Physiol 46 1177–1186

    Google Scholar 

  • G.M. Church W. Gilbert (1984) ArticleTitleGenomic sequencing Proc Natl Acad Sci USA 81 1991–1995 Occurrence Handle1:CAS:528:DyaL2cXkt1Sit7k%3D Occurrence Handle6326095

    CAS  PubMed  Google Scholar 

  • D.J. Cosgrove (1999) ArticleTitleEnzymes and other agents that enhance cell wall extensibility Annu Rev Plant Physiol Plant Mol Biol 50 391–417 Occurrence Handle10.1146/annurev.arplant.50.1.391 Occurrence Handle1:CAS:528:DyaK1MXkt1yksbg%3D Occurrence Handle11541953

    Article  CAS  PubMed  Google Scholar 

  • J. Daie (1987) ArticleTitleSucrose uptake in isolated phloem of celery is a single saturable transport system Planta 171 474–482

    Google Scholar 

  • C.P. Darley A.M. Forrester S.J. Mc Queen-Mason (2001) ArticleTitleThe molecular basis of plant cell wall extension Plant Mol Biol 47 179–195 Occurrence Handle10.1023/A:1010687600670 Occurrence Handle1:CAS:528:DC%2BD3MXmslGmsbs%3D Occurrence Handle11554471

    Article  CAS  PubMed  Google Scholar 

  • F.A. Daudet A. Lacointe J.P. Gaudillere P. Cruiziat (2002) ArticleTitleGeneralized Münch coupling between sugar and water fluxes for modelling carbon allocation as affected by water status J Theor Biol 214 481–498

    Google Scholar 

  • L. Diatchenko Y.F. Lau A.P Campbell A. Chenchik F. Moqadam B. Huang S. Lukyanov K. Lukyanov N Gurskaya E.D. Sverdlov P.D. Siebert (1996) ArticleTitleSuppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries Proc Natl Acad Sci USA 93 6025–6030 Occurrence Handle10.1073/pnas.93.12.6025 Occurrence Handle1:CAS:528:DyaK28Xjs1Ojsbk%3D Occurrence Handle8650213

    Article  CAS  PubMed  Google Scholar 

  • S. Dinant A.M. Clark Y. Zhu F. Vilaine JC. Palauqui C. Kusiak G.A. Thompson (2003) ArticleTitleDiversity of the superfamily of phloem lectins (phloem protein 2) in angiosperms Plant Physiol 131 114–128

    Google Scholar 

  • J.T. Dongen Particlevan U. Schurr M. Pfister P. Geigenberger (2003) ArticleTitlePhloem metabolism and function have to cope with low internal oxygen Plant Physiol 131 1529–1543

    Google Scholar 

  • S. Draghici (2003) Methods for selecting differentially regulated genes S. Draghici (Eds) Data Analysis Tools for DNA Microarrays. Chapman & Hall/CRC London 341–362

    Google Scholar 

  • C. Ellis I. Karafyllidis J.G. Turner (2002) ArticleTitleConstitutive activation of jasmonate signaling in an Arabidopsis mutant correlates with enhanced resistance to Erysiphe cichoracearum, Pseudomonas syringae, and Myzus persicae Mol Plant Microbe Interact 15 1025–1030

    Google Scholar 

  • M.T. Esquerre-Tugaye G. Boudart B. Dumas (2000) ArticleTitleCell wall degrading enzymes, inhibitory proteins, and oligosaccharides participate in the molecular dialogue between plants and pathogens Plant Physiol Biochem 38 157–163

    Google Scholar 

  • J.D. Everard R. Gucci S.C. Kann J.A. Flore W.H. Loescher (1994) ArticleTitleGas exchange and carbon partitioning in the leaves of celery (Apium graveolens L.) at various levels of root zone salinity Plant Physiol 106 281–292 Occurrence Handle1:CAS:528:DyaK2cXmtVSqurc%3D Occurrence Handle12232328

    CAS  PubMed  Google Scholar 

  • N. Frangne M. Maeshima A.R. Schaffner T. Mandel E. Martinoia J.L. Bonnemain (2001) ArticleTitleExpression and distribution of a vacuolar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes Planta 212 270–278

    Google Scholar 

  • C.A. Frye D. Tang R.W. Innes (2001) ArticleTitleNegative regulation of defense responses in plants by a conserved MAPKK kinase Proc Natl Acad Sci USA 98 373–378

    Google Scholar 

  • J.A. Gatehouse (2002) ArticleTitlePlant resistance towards insect herbivores: a dynamic interaction New Phytologist 156 145–169

    Google Scholar 

  • C. Girousse M. Faucher C. Kleinpeter J.L. Bonnemain (2003) ArticleTitleDissection of the effect of the aphid Acyrthosiphon pisum feeding on assimilate partitioning in Medicago sativa New Phytologist 157 83–92

    Google Scholar 

  • J.A. Godoy R. Lunar S. Torres-Schumann J. Moreno R.M. Rodrigo J.A. Pintor-Toro (1994) ArticleTitleExpression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants Plant Mol Biol 26 1921–1934

    Google Scholar 

  • M. Goellner X. Wang E.L. Davis (2001) ArticleTitleEndo-beta-1,4-glucanase expression in compatible plant-nematode interactions Plant Cell 13 2241–2255

    Google Scholar 

  • W.J. Guo W. Bundithya P.B. Goldsbrough (2003) ArticleTitleCharacterization of the Arabidopsis metallothionein gene family: tissue-specific expression and induction during senescence and in response to copper New Phytologist 159 369–381

    Google Scholar 

  • B.K. Hale J.S. Bale J. Pritchard G.J. Masters V.K. Brown (2003) ArticleTitleEffects of host plant drought stress on the performance of the bird cherry-oat aphid, Rhopalosiphum padi (L.): a mechanistic analysis Ecol Entomol 28 666–677 Occurrence Handle10.1111/j.1365-2311.2003.00563.x

    Article  Google Scholar 

  • M. Hertzberg H. Aspeborg J. Schrader A. Andersson R. Erlandsson K. Blomqvist R. Bhalerao M. Uhlen T.T. Teeri J. Lundeberg B. Sundberg P. Nilsson G. Sandberg (2001) ArticleTitleA transcriptional roadmap to wood formation Proc Natl Acad Sci USA 98 14732–14737

    Google Scholar 

  • D. Hui J. Iqbal K. Lehmann K. Gase H.P. Saluz I.T. Baldwin (2003) ArticleTitleMolecular Interactions between the specialist herbivore Manduca sexta (Lepidoptera, Sphingidae) and its natural host Nicotiana attenuata: V. Microarray analysis and further characterization of large-scale changes in herbivore-induced mRNAs Plant Physiol 131 1877–1893 Occurrence Handle10.1104/pp.102.018176 Occurrence Handle1:CAS:528:DC%2BD3sXjt1Sqtr8%3D Occurrence Handle12692347

    Article  CAS  PubMed  Google Scholar 

  • N. Ivashikina R. Deeken P. Ache E. Kranz B. Pommerrenig N. Sauer R. Hedrich (2003) ArticleTitleIsolation of AtSUC2 promoter-GFP-marked companion cells for patch-clamp studies and expression profiling Plant J 36 931–945

    Google Scholar 

  • M.K. Kerr M. Martin G.A. Churchill (2000) ArticleTitleAnalysis of variance for gene expression microarray data J Comput Biol 7 819–837

    Google Scholar 

  • A. Kessler I.T. Baldwin (2003) ArticleTitlePlant responses to insect herbivory: the emerging molecular analysis Annu Rev Plant Biol 53 299–328

    Google Scholar 

  • H.H. Kirch R. Vera-Estrella D. Golldack F. Quigley C.B. Michalowski B.J. Barkla H.J. Bohnert (2000) ArticleTitleExpression of water channel proteins in Mesembryanthemum crystallinum Plant Physiol 123 111–124

    Google Scholar 

  • S. Lee S.Y. Kim E. Chung Y.H. Joung H.S. Pai C.G. Hur D. Choi (2004) ArticleTitleEST and microarray analyses of pathogen-responsive genes in hot pepper (Capsicum annuum L.) non-host resistance against soybean pustule pathogen (Xanthomonas axonopodispv. glycines) Funct Integr Genomics 4 196–205 Occurrence Handle10.1007/s10142-003-0099-1 Occurrence Handle14760538

    Article  PubMed  Google Scholar 

  • T.B. Macedo C.S. Bastos L.G. Higley K.R. Ostlie S. Madhavan (2003) ArticleTitlePhotosynthetic responses of soybean to soybean aphid (Homoptera: Aphididae) injury J Econ Entomol 96 188–193

    Google Scholar 

  • J. Malamy P. Sanchez-Casas J. Hennig A. Guo D.F. Klessig (1996) ArticleTitleDissection of the salicylic acid signalling pathway in tobacco Mol Plant-Microbe Interact 9 474–482

    Google Scholar 

  • O. Martinezde Iladuya Q.G. Xie I. Kaloshian (2003) ArticleTitleAphid-induced defense responses in Mi-1-mediated compatible and incompatible tomato interactions Mol Plant-Microbe Interact 16 699–708

    Google Scholar 

  • P.W. Miles (1999) ArticleTitleAphid saliva Biol Rev 74 41–85

    Google Scholar 

  • P.W. Miles J.J. Oertli (1993) ArticleTitleThe significance of antioxidants in the aphid–plant interaction: the redox hypothesis Entomol Exp Appl 67 275–283

    Google Scholar 

  • G. Mir J. Domenech G. Huguet W.J. Guo P. Goldsbrough S. Atrian M. Molinas (2004) ArticleTitleA plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress J Exp Bot 55 2483–2493

    Google Scholar 

  • H. Mira F. Martinez-Garcia L. Penarrubia (2001) ArticleTitleEvidence for the plant-specific intercellular transport of the Arabidopsis copper chaperone CCH Plant J 25 521–528

    Google Scholar 

  • P.J. Moran G.A. Thompson (2001) ArticleTitleMolecular responses to aphid feeding in Arabidopsis in relation to plant defense pathways Plant Physiol 125 1074–1085 Occurrence Handle10.1104/pp.125.2.1074 Occurrence Handle1:CAS:528:DC%2BD3MXhs1Klt7Y%3D Occurrence Handle11161062

    Article  CAS  PubMed  Google Scholar 

  • P.J. Moran Y. Cheng J.L. Cassell G.A. Thompson (2002) ArticleTitleGene expression profiling of Arabidopsis thaliana in compatible plant-aphid interactions Arch. Insect Biochem. Physiol 51 182–203

    Google Scholar 

  • E. Münch (1930) Die Stoffbewegungen in der Pflanze Gustav Fisher Jena

    Google Scholar 

  • M. Nakazono F. Qiu L.A. Borsuk P.S. Schnable (2003) ArticleTitleLaser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize Plant Cell 15 583–596 Occurrence Handle10.1105/tpc.008102 Occurrence Handle1:CAS:528:DC%2BD3sXisVektL4%3D Occurrence Handle12615934

    Article  CAS  PubMed  Google Scholar 

  • X. Ni S.S. Quisenberry T. Heng-Moss J. Markwell G. Sarath R. Klucas F. Baxendale (2001) ArticleTitleOxidative responses of resistant and susceptible cereal leaves to symptomatic and nonsymptomatic cereal aphid (Hemiptera: Aphididae) feeding J Econ Entomol 94 743–751

    Google Scholar 

  • N. Noiraud S. Delrot R. Lemoine (2000) ArticleTitleThe sucrose transporter of celery. Identification and expression during salt stress Plant Physiol 122 1447–1455 Occurrence Handle10.1104/pp.122.4.1447 Occurrence Handle1:CAS:528:DC%2BD3cXktFWjs7w%3D Occurrence Handle10759540

    Article  CAS  PubMed  Google Scholar 

  • N. Noiraud L. Maurousset R. Lemoine (2001) ArticleTitleIdentification of a mannitol transporter, AgMaT1, in celery phloem Plant Cell 13 695–705

    Google Scholar 

  • M. Nylander J. Svensson E.T. Palva B.V. Welin (2001) ArticleTitleStress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana Plant Mol Biol 45 263–279

    Google Scholar 

  • K.J. Oparka R. Turgeon (1999) ArticleTitleSieve elements and companion cells – traffic control centers of the phloem Plant Cell 11 739–750 Occurrence Handle10.1105/tpc.11.4.739 Occurrence Handle1:CAS:528:DyaK1MXjtFSru74%3D Occurrence Handle10213790

    Article  CAS  PubMed  Google Scholar 

  • C.J. Park K.J. Kim R. Shin J.M. Park Y.C. Shin K.H. Paek (2004) ArticleTitlePathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway Plant J 37 186–198

    Google Scholar 

  • M. Petersen P. Brodersen H. Naested E. Andreasson U. Lindhart B. Johansen H.B. Nielsen M. Lacy M.J. Austin J.E. Parker S.B. Sharma D.F. Klessig R. Martienssen O. Mattsson A.B. Jensen J. Mundy (2000) ArticleTitleArabidopsis MAP kinase 4 negatively regulates systemic acquired resistance Cell 103 1111–1120 Occurrence Handle10.1016/S0092-8674(00)00213-0

    Article  Google Scholar 

  • K.L. Ponder J. Pritchard R. Harrington J.S. Bale (2001) ArticleTitleFeeding behaviour of the aphid Rhopalosiphum padi (Hemiptera: Aphididae) on nitrogen and water-stressed barley (Hordeum vulgare) seedlings Bull Entomol Res 91 125–130

    Google Scholar 

  • J. Pritchard S. Winch N. Gould (2000) ArticleTitlePhloem water relations and root growth Aust J Plant Physiol 27 539–548

    Google Scholar 

  • P. Reymond H. Weber M. Damond E.E. Farmer (2000) ArticleTitleDifferential gene expression in response to mechanical wounding and insect feeding in Arabidopsis Plant Cell 12 707–720 Occurrence Handle10.1105/tpc.12.5.707 Occurrence Handle1:CAS:528:DC%2BD3cXjvFyhurk%3D Occurrence Handle10810145

    Article  CAS  PubMed  Google Scholar 

  • T. Roitsch (1999) ArticleTitleSource-sink regulation by sugar and stress Curr Opin Plant Biol 2 198–206 Occurrence Handle10.1016/S1369-5266(99)80036-3

    Article  Google Scholar 

  • J. Sandström A. Telang N.A. Moran (2000) ArticleTitleNutritional enhancement of host plants by aphids – a comparison of three aphid species on grasses J Insect Physiol 46 33–40

    Google Scholar 

  • A.R. Schäffner (1998) ArticleTitleAquaporin function, structure, and expression: are there more surprises to surface in water relations? Planta 204 131–139 Occurrence Handle10.1007/s004250050239 Occurrence Handle9487723

    Article  PubMed  Google Scholar 

  • P.M. Schenk K. Kazan J.M. Manners J.P. Anderson R.S. Simpson I.W. Wilson S.C. Somerville D.J. Maclean (2003) ArticleTitleSystemic gene expression in Arabidopsis during an incompatible interaction with Alternaria brassicicola Plant Physiol 132 999–1010

    Google Scholar 

  • G. Sherlock (2000) ArticleTitleAnalysis of large-scale gene expression data Curr Opin Immunol 12 201–205

    Google Scholar 

  • R.D. Sjölund (1997) ArticleTitleThe phloem sieve element: a river runs through it Plant Cell 9 1137–1146

    Google Scholar 

  • D.Y. Sung C.L. Guy (2003) ArticleTitlePhysiological and molecular assessment of altered expression of Hsc70–1 in Arabidopsis. Evidence for pleiotropic consequences Plant Physiol 132 979–987

    Google Scholar 

  • J.E. Taylor P.E. Hatcher N.D. Paul (2004) ArticleTitleCrosstalk between plant responses to pathogens and herbivores: a view from the outside in J Exp Bot 55 159–168

    Google Scholar 

  • B.P. Thomma B.P. Cammue K. Thevissen (2002) ArticleTitlePlant defensins Planta 216 193–202

    Google Scholar 

  • A.J.E. Van Bel (2003) ArticleTitleThe phloem, a miracle of ingenuity Plant Cell Environ 26 125–149

    Google Scholar 

  • A.J. Van Bel K. Ehlers M. Knoblauch (2002) ArticleTitleSieve elements caught in the act Trends Plant Sci 7 126–132

    Google Scholar 

  • I. Vercauteren J. Almeida Engler Particlede R. Groodt Particlede G. Gheysen (2002) ArticleTitleAn Arabidopsis thaliana pectin acetylesterase gene is upregulated in nematode feeding sites induced by root-knot and cyst nematodes Mol Plant Microbe Interact 15 404–407

    Google Scholar 

  • P. Veronese M.T. Ruiz M.A. Coca A. Hernandez-Lopez H. Lee J.I. Ibeas B. Damsz J.M. Pardo P.M. Hasegawa R.A. Bressan Narasimhan M.L. (2003) ArticleTitleIn defense against pathogens. Both plant sentinels and foot soldier need to know the enemy Plant Physiol 131 1580–1590

    Google Scholar 

  • T.C. Verwoerd M.M. Dekker A. Hoekema (1989) ArticleTitleA small-scale procedure for the rapid isolation of plant RNAs Nucl Acids Res 17 2362

    Google Scholar 

  • F. Vilaine J.C. Palauqui J. Amselem C. Kusiak R. Lemoine S. Dinant (2003) ArticleTitleToward phloem deciphering: a transcriptome analysis of the phloem of Apium graveolens Plant J. 36 67–81

    Google Scholar 

  • C. Voelckel I.T. Baldwin (2003) ArticleTitleDetecting herbivore-specific transcriptional responses in plants with multiple DDRT-PCR and subtractive library procedures Physiol. Plantarum 118 240–252

    Google Scholar 

  • C. Voelckel W.W. Weisser I.T. Baldwin (2004) ArticleTitleAn analysis of plant–aphid interactions by different microarray hybridization strategies Mol Ecol 13 3187–3195

    Google Scholar 

  • S. Vorwerk S. Somerville C. Somerville (2004) ArticleTitleThe role of plant cell wall polysaccharide composition in disease resistance Trends Plant Sci 9 203–209 Occurrence Handle1:CAS:528:DC%2BD2cXivVGjsbo%3D Occurrence Handle15063871

    CAS  PubMed  Google Scholar 

  • L.L. Walling (2000) ArticleTitleThe myriad plant responses to herbivores J Plant Growth Reg 19 195–216 Occurrence Handle1:CAS:528:DC%2BD3cXnvF2hu7w%3D Occurrence Handle11038228

    CAS  PubMed  Google Scholar 

  • C. Walz P. Giavalisco M. Schad M. Juenger J. Klose J. Kehr (2004) ArticleTitleProteomics of curcurbit phloem exudate reveals a network of defence proteins Phytochemistry 65 1795–1804

    Google Scholar 

  • Y. Wu E.T. Thorne R.E. Sharp D.J. Cosgrove (2001) ArticleTitleModification of expansin transcript levels in the maize primary root at low water potentials Plant Physiol 126 1471–1479

    Google Scholar 

  • F. Zhang L. Zhu G. He (2004) ArticleTitleDifferential gene expression in response to brown planthopper feeding in rice J Plant Physiol 161 53–62 Occurrence Handle1:CAS:528:DC%2BD2cXis1eiurY%3D Occurrence Handle15002664

    CAS  PubMed  Google Scholar 

  • K. Zhu-Salzman R. Salzman J.E. Ahn H. Koiwa (2004) ArticleTitleTranscriptional regulation of sorghum defense determinants againt a phloem-feeding aphid Plant Physiol 134 420–431 Occurrence Handle10.1104/pp.103.028324 Occurrence Handle1:CAS:528:DC%2BD2cXhtVansrY%3D Occurrence Handle14701914

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Sylvie Dinant.

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EST, macroarray and clustering data are available from our website [http://www-biocel.versailles.inra.fr/phloem]. Data deposition: The sequences reported in this paper have been deposited in the Genbank database (Accession nos.: AY607692-AY607700, AY611007, CN253939-CN255151, CV512445-CV512447 and CV651120-CV651121).

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Divol, F., Vilaine, F., Thibivilliers, S. et al. Systemic response to aphid infestation by Myzus persicae in the phloem of Apium graveolens. Plant Mol Biol 57, 517–540 (2005). https://doi.org/10.1007/s11103-005-0338-z

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