Plant Molecular Biology

, Volume 56, Issue 6, pp 947–957

Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I

  • Mariano Perales
  • Gustavo Parisi
  • María Silvina Fornasari
  • Alejandro Colaneri
  • Fernando Villarreal
  • Nahuel González-Schain
  • Julián Echave
  • Diego Gómez-Casati
  • Hans-Peter Braun
  • Alejandro Araya
  • Eduardo Zabaleta
Article

DOI: 10.1007/s11103-004-6324-z

Cite this article as:
Perales, M., Parisi, G., Fornasari, M.S. et al. Plant Mol Biol (2004) 56: 947. doi:10.1007/s11103-004-6324-z

Abstract

We report the identification by two hybrid screens of two novel similar proteins, called Arabidopsis thaliana gamma carbonic anhydrase like1 and 2 (AtγCAL1 and AtγCAL2), that interact specifically with putative Arabidopsis thaliana gamma Carbonic Anhydrase (AtγCA) proteins in plant mitochondria. The interaction region that was located in the N-terminal 150 amino acids of mature AtγCA and AtγCA like proteins represents a new interaction domain. In vitro experiments indicate that these proteins are imported into mitochondria and are associated with mitochondrial complex I as AtγCAs. All plant species analyzed contain both AtγCA and AtγCAL sequences indicating that these genes were conserved throughout plant evolution. Structural modeling of AtγCAL sequences show a deviation of functionally important active site residues with respect to γCAs but could form active interfaces in the interaction with AtγCAs. We postulate a CA complex tightly associated to plant mitochondrial complex.

Keywords

Complex IGamma carbonic anhydraseplant mitochondriarespiratory chain

Copyright information

© Springer 2005

Authors and Affiliations

  • Mariano Perales
    • 1
  • Gustavo Parisi
    • 2
  • María Silvina Fornasari
    • 2
  • Alejandro Colaneri
    • 1
  • Fernando Villarreal
    • 1
  • Nahuel González-Schain
    • 1
  • Julián Echave
    • 2
  • Diego Gómez-Casati
    • 1
  • Hans-Peter Braun
    • 3
  • Alejandro Araya
    • 4
  • Eduardo Zabaleta
    • 1
    • 5
  1. 1.Instituto de Investigaciones BiotecnológicasIIB-INTECH (CONICET/UNSAM)ChascomúsArgentina
  2. 2.Centro de Estudios de InvestigacionesUniversidad Nacional de QuilmesBernal.Argentina
  3. 3.Institut für Angewandte GenetikUniversität HannoverHannoverGermany
  4. 4.UMR 5097 R.E.G.E.R.CNRS and Université Victor Segalen Bordeaux 2BordeauxFrance
  5. 5.Instituto de Investigaciones BiológicasUniversidad Nacional de Mar del PlataMar del PlataArgentina