Plant Molecular Biology

, Volume 56, Issue 1, pp 29–55

Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray

Authors

  • Seiji Takahashi
    • Laboratory of Plant Molecular BiologyRIKEN Tsukuba Institute
  • Motoaki Seki
    • Laboratory of Plant Molecular BiologyRIKEN Tsukuba Institute
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
  • Junko Ishida
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
  • Masakazu Satou
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
    • Genomic Knowledge Base Research Team, Bioinformatics GroupRIKEN Genomic Science Centre
  • Tetsuya Sakurai
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
    • Genomic Knowledge Base Research Team, Bioinformatics GroupRIKEN Genomic Science Centre
  • Mari Narusaka
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
  • Asako Kamiya
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
  • Maiko Nakajima
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
  • Akiko Enju
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
  • Kenji Akiyama
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
    • Genomic Knowledge Base Research Team, Bioinformatics GroupRIKEN Genomic Science Centre
  • Kazuko Yamaguchi-Shinozaki
    • Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ministry of AgricultureForestry, and Fisheries
  • Kazuo Shinozaki
    • Laboratory of Plant Molecular BiologyRIKEN Tsukuba Institute
    • Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences CenterRIKEN Yokohama Institute
Article

DOI: 10.1007/s11103-004-2200-0

Cite this article as:
Takahashi, S., Seki, M., Ishida, J. et al. Plant Mol Biol (2004) 56: 29. doi:10.1007/s11103-004-2200-0

Abstract

Transcriptional regulation in response to hyperosmotic, high-salinity and oxidative stress, and abscisic acid (ABA) treatment in Arabidopsis suspension-cultured cell line T87 was investigated with a cDNA microarray containing 7000 independent full-length Arabidopsis cDNAs. The transcripts of 102, 11, 84 and 73 genes were increased more than 5-fold within 5h after treatment with 0.5M mannitol, 0.1M NaCl, 50μM ABA and 10mM H2O2, respectively. On the other hand, the transcripts of 44, 57, 25 and 34 genes were down-regulated to less than one-third within 5h after treatment with 0.5M mannitol, 0.1M NaCl, 50μM ABA and 10mM H2O2, respectively. Venn diagram analysis revealed 11 genes were induced significantly by mannitol, NaCl, and ABA, indicating crosstalk among these signaling pathways. Comparison of the genes induced by each stress revealed that 32%, 17% and 33% of mannitol-, NaCl- and ABA-inducible genes were also induced by H2O2, indicating the crosstalk between the signaling pathways for osmotic stress and oxidative stress. Although the expression profiles revealed that the T87 cells had most of the regulatory systems seen in Arabidopsis seedlings, the T87 cells did not have one of ABA-dependent signaling pathways.

abiotic stress Arabidopsis thaliana cDNA microarray full-length cDNA T87 cell culture

Supplementary material

TABLE_S1.XLS (254 kb)
Table S1 (XLS 255 KB)
TABLE_S2.XLS (102 kb)
Table S2 (XLS 103 KB)
TABLE_S3.XLS (162 kb)
Table S3 (XLS 163 KB)
TABLE_S4.XLS (100 kb)
Table S4 (XLS 101 KB)
TABLE_S5-1.XLS (38 kb)
Table S5-1 (XLS 39 KB)
TABLE_S5-2.XLS (74 kb)
Table S5-2 (XLS 74 KB)
TABLE_S6-1.XLS (28 kb)
Table S6-1 (XLS 29 KB)
TABLE_S6-2.XLS (96 kb)
Table S6-2 (XLS 97 KB)
TABLE_S7-1.XLS (13 kb)
Table S7-1 (XLS 13 KB)
TABLE_S7-2.XLS (58 kb)
Table S7-2 (XLS 58 KB)
TABLE_S8-1.XLS (22 kb)
Table S8-1 (XLS 22 KB)
TABLE_S8-2.XLS (57 kb)
Table S8-2 (XLS 57 KB)

Copyright information

© Kluwer Academic Publishers 2004