Plant Molecular Biology

, Volume 55, Issue 3, pp 399–416

Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley


  • Caterina Marè
    • Istituto Sperimentale per la Cerealicoltura
  • Elisabetta Mazzucotelli
    • Istituto Sperimentale per la Cerealicoltura
    • Dipartimento di Biologia sez. Biologia evolutivaUniversità degli Studi di Ferrara
  • Cristina Crosatti
    • Istituto Sperimentale per la Cerealicoltura
  • Enrico Francia
    • Istituto Sperimentale per la Cerealicoltura
  • A.␣michele Stanca
    • Istituto Sperimentale per la Cerealicoltura
  • Luigi Cattivelli
    • Istituto Sperimentale per la Cerealicoltura

DOI: 10.1007/s11103-004-0906-7

Cite this article as:
Marè, C., Mazzucotelli, E., Crosatti, C. et al. Plant Mol Biol (2004) 55: 399. doi:10.1007/s11103-004-0906-7


WRKY proteins constitute a large family of plant specific transcription factors implicated in many different processes. Here we describe Hv-WRKY38,a barley gene coding for a WRKY protein, whose expression is involved in cold and drought stress response. Hv-WRKY38 was early and transiently expressed during exposure to low non-freezing temperature, in ABA-independent manner. Furthermore, it showed a continuous induction during dehydration and freezing treatments. A WRKY38:YFP fusion protein was found to localise into the nucleus upon introduction into epidermal onion cells. Bacterially expressed Hv-WRKY38 was able to bind in vitro to the W-box element (T)TGAC(C/T) also recognisable by other WRKY proteins. Hv-WRKY38genomic DNA was sequenced and mapped onto the centromeric region of the barley chromosome 6H. Arabidopsis and rice sequences homologous to Hv-WRKY38 were also identified. Our results indicate that Hv-WRKY38 transcription factor may play a regulatory role in abiotic stress response.

barleycolddroughtfreezingtranscription factorWRKY protein

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© Kluwer Academic Publishers 2004