Plant Molecular Biology

, Volume 55, Issue 2, pp 253–262

Modulation of flowering responses in different Nicotiana varieties

  • Petr Smykal
  • Roland Gleissner
  • Laurent Corbesier
  • Klaus Apel
  • Siegbert Melzer
Article

DOI: 10.1007/s11103-004-0557-8

Cite this article as:
Smykal, P., Gleissner, R., Corbesier, L. et al. Plant Mol Biol (2004) 55: 253. doi:10.1007/s11103-004-0557-8

Abstract

We have identified and characterized a FLOWERING PROMOTING FACTOR 1(FPF1) gene from tobacco (NtFPF1). Over-expression of NtFPF1 leads to early flowering in the day-neutral tobacco Nicotiana tabacum cv. Hicks, and under inductive photoperiods also in the short-day Nicotiana tabacum cv. Hicks Maryland Mammoth (MM) tobacco and the long-day plant Nicotiana sylvestris. N. sylvestris wild-type plants remained in the rosette stage and never flowered under non-inductive short-days, whereas 35S::NtFPF1 transgenic plants bolted but did not flower. However, if treated with gibberellins, transgenic N. sylvestrisplants flowered much faster under non-inductive short days than corresponding wild type plants, indicating an additive effect of gibberellins and the NtFPF1 protein in flowering time control. The day-neutral wild type cv. Hicks and the short-day cv. Hicks MM plants exhibit an initial rosette stage, both under short- and long-days. In the transgenic lines, this rosette stage was completely abolished. Wild-type plants of cv. Hicks MM never flowered under long days; however, all transgenic lines over-expressing NtFPF1 flowered under this otherwise non-inductive photoperiod.

Floral inductionFlowering timeFPF1GibberellinTobacco

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Petr Smykal
    • 1
  • Roland Gleissner
    • 1
  • Laurent Corbesier
    • 1
    • 2
  • Klaus Apel
    • 1
  • Siegbert Melzer
    • 1
    • 1
  1. 1.Swiss Federal Institute of Technology Zürich (ETH)Institute of Plant SciencesUniversitaetstrasse 2Switzerland; Department of Biotechnology, AGRITEC Plant Research Ltd., Zemedelska 2520/16 787 01, Czech Republic
  2. 2.Max-Planck-Institute for Plant Breeding ResearchKöln, Germany