Article

Pituitary

, Volume 16, Issue 1, pp 83-90

First online:

Involvement of genes related to inflammation and cell cycle in Idiopathic Short Stature

  • Letizia TrovatoAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte OrientaleLaboratory of Molecular and Cellular Endocrinology, Department of Internal Medicine, University of Turin Email author 
  • , Flavia ProdamAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale
  • , Giulia GenoniAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale
  • , Francesca De RienzoAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale
  • , Gillian E. WalkerAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale
  • , Stefania MoiaAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale
  • , Stefania RiccomagnoAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale
  • , Simonetta BelloneAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale
  • , Gianni BonaAffiliated withDivision of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Idiopathic Short Stature (ISS) defines a condition in which height is <−2SD compared to the mean of a reference population where systemic, endocrinological, nutritional or chromosomal disorders have not been identified and diagnosis is based on exclusion of any known causes of short stature. JAK/STAT pathway is triggered by GH binding to the GH receptor and promotes cellular growth through transcription of GH-responsive genes. In order to identify “candidate genes” differently expressed in ISS subjects with respect to control ones, we analyzed the expression of 84 genes related to JAK/STAT pathway by RT2 Profiler PCR array approach in a total of 10 subjects. Then, we validated the observed data by Real Time PCR and ELISA assays in a major number of subjects. We found two genes that were differently expressed in ISS subjects with respect to the control group: CXCL9 and FCGR1A/CD64, both significantly up-regulated (fold change 2.17 and 1.70, respectively) and belonging to family of IFN-γ-inducible factors. Further, ISS subjects showed an increased gene expression of IFN-γ and IFI16, higher serum levels of IFN-γ but similar levels of CXCL9 when compared to healthy subjects. In addition, we showed a pubertal modulation of CXCL9 levels. These data suggest that inflammatory and regulatory factors of the cell cycle may be involved in the ISS condition, introducing a new perspective to its etiology.

Keywords

Cytokines Chemokines Gene profile Idiopathic short stature Cellular cycle