Pituitary

, Volume 9, Issue 2, pp 109–120

Phosphoproteomic analysis of the human pituitary

  • Sarka Beranova-Giorgianni
  • Yingxin Zhao
  • Dominic M. Desiderio
  • Francesco Giorgianni
Article

DOI: 10.1007/s11102-006-8916-x

Cite this article as:
Beranova-Giorgianni, S., Zhao, Y., Desiderio, D.M. et al. Pituitary (2006) 9: 109. doi:10.1007/s11102-006-8916-x

Abstract

The pituitary is the central endocrine gland that regulates the functions of various target organs in the human body. Because of the pivotal regulatory role of the pituitary, it is essential to define on a global scale the components of the pituitary protein machinery, including a comprehensive characterization of the post-translational modifications of the pituitary proteins. Of particular interest is the examination of the phosphorylation status of the pituitary in health and disease. Towards the goal of global profiling of pituitary protein phosphorylation, we report here the application of the in-gel IEF-LC-MS/MS approach to the study of the pituitary phosphoproteome. The analytical strategy combined isoelectric focusing in immobilized pH gradient strips with immobilized metal ion affinity chromatography and mass spectrometry. With this method, a total of 50 phosphorylation sites were characterized in 26 proteins. Because the investigation involved primary tissue, the findings provide a direct glimpse into the phosphoprotein machinery operating within the human pituitary tissue microenvironment.

Keywords

Human pituitary tissue Isoelectric focusing Mass spectrometry Phosphoproteome 

Supplementary material

supp.doc (108 kb)

Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Sarka Beranova-Giorgianni
    • 1
    • 2
    • 3
  • Yingxin Zhao
    • 2
  • Dominic M. Desiderio
    • 1
    • 3
  • Francesco Giorgianni
    • 1
    • 3
  1. 1.Charles B. Stout Neuroscience Mass Spectrometry LaboratoryThe University of Tennessee Health Science CenterMemphisUSA
  2. 2.Department of Pharmaceutical SciencesThe University of Tennessee Health Science CenterMemphisUSA
  3. 3.Department of NeurologyThe University of Tennessee Health Science Center MemphisUSA