Article

Phytochemistry Reviews

, Volume 7, Issue 3, pp 525-537

Metabolomics: back to basics

  • R. VerpoorteAffiliated withDivision of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University Email author 
  • , Y. H. ChoiAffiliated withDivision of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
  • , N. R. MustafaAffiliated withDivision of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University
  • , H. K. KimAffiliated withDivision of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Metabolomics has developed into a major tool in functional genomics and plant systems biology. The various methods used for metabolomic analysis will be discussed from the analytical methods back to the preanalytical phase and the biological experiment. Particularly aspects of the preanalytical phase of the analysis is dealt with, including the risks of artefact formation with the various commonly used solvents. Metabolomics is like a snap shot, and conclusions from dynamic systems must be drawn with great care as demonstrated with a biosynthetic study of salicylate in Catharanthus roseus cell cultures.

Keywords

Metabolomics NMR spectrometry Sample preparation Extraction Artefacts Catharanthus roseus