Phytochemistry Reviews

, Volume 6, Issue 2, pp 363–381

Cellular and sub-cellular organisation of the monoterpenoid indole alkaloid pathway in Catharanthus roseus

ORIGINAL PAPER

DOI: 10.1007/s11101-006-9017-1

Cite this article as:
Mahroug, S., Burlat, V. & St-Pierre, B. Phytochem Rev (2007) 6: 363. doi:10.1007/s11101-006-9017-1

Abstract

The Madagascar periwinkle (Catharanthus roseus) monoterpene indole alkaloids (MIAs), either as (hetero)dimers (vinblastine, vincristine) or as monomers (ajmalicine) are valuable pharmaceuticals with antitumoral and hypotensive properties. The biosynthetic pathway of these secondary metabolites involves several subpathways (so-called indole, methylerythritol phosphate, secoiridoid-monoterpenoids, and the MIA pathway itself). So far, 16 cDNA sequences with direct involvement in the pathway have been obtained and some additional enzymatic activities have been characterised. Metabolomic, transcriptomic and proteomic studies have revealed that this pathway presents a high degree of spatial organisation at the organ, cellular and sub-cellular levels. This paper reviews the evidence on the spatial organisation of the MIA pathway at various levels of resolution in terms of the following topics. (1) The organ-specific distribution of MIAs, related genes, related enzymes and related enzymatic activities, mainly focussing on repartition in roots versus aerial organs. (2) The cell-specific occurrence of MIA-related gene expression identified by in situ hybridisation and corresponding protein accumulation identified by immunolocalisation, with particular emphasis on the well-characterised multi-cellular compartmentation in aerial organs. In situ hybridisation revealed that the nine transcripts studied could be classified into three populations according to their position in the pathway (early-, intermediate- and late-step genes) and to their cell-specific expression patterns (internal phloem parenchyma, epidermis, laticifers/idioblasts). (3) The still poorly characterised sub-cellular localisation of MIA-related enzymes. Finally, these results are compared with the situation in other families producing other types of alkaloids, and future prospects are discussed.

Keywords

CompartmentationImmunocytolabellingIn situ hybridisationMicroscopySecondary metabolism

Abbreviations

ABC

ATP-binding cassette

ER

endoplasmic reticulum

GFP

green fluorescent protein

GUS

β-glucuronidase

MEP

methylerythritol phosphate

MIA

monoterpene indole alkaloid

TEM

transmission electron microscopy

UV

ultra violet

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Samira Mahroug
    • 1
    • 2
  • Vincent Burlat
    • 1
    • 2
  • Benoit St-Pierre
    • 1
    • 2
  1. 1.EA 2106 “Biomolécules et Biotechnologies Végétales”, UFR Sciences et TechniquesUniversité François-Rabelais de ToursToursFrance
  2. 2.Unité sous Contrat reconnue par l’INRA, USC 2021 “Facteurs de Transcription et Ingénierie Métabolique Végétale”Université François-Rabelais de ToursToursFrance