Photosynthetica

, Volume 48, Issue 3, pp 409–416

Antisense-mediated suppression of tomato zeaxanthin epoxidase alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance

  • N. Wang
  • B. Li
  • H. -L. Feng
  • Q. -Y. Zhang
  • X. -H. Yang
  • Q. -W. Meng
Original Papers

DOI: 10.1007/s11099-010-0053-0

Cite this article as:
Wang, N., Li, B., Feng, H.L. et al. Photosynthetica (2010) 48: 409. doi:10.1007/s11099-010-0053-0

Abstract

A tomato (Lycopersicon esculentum Mill.) zeaxanthin epoxidase gene (LeZE) was isolated and antisense transgenic tomato plants were produced. Northern, southern, and western blot analyses demonstrated that antisense LeZE was transferred into the tomato genome and the expression of LeZE was inhibited. The ratio of (A+Z)/(V+A+Z) in antisense transgenic plants was maintained at a higher level than in the wild type (WT) plants under high light and chilling stress with low irradiance. The value of non-photochemical quenching (NPQ) in WT and transgenic plants was not affected during the stresses. The oxidizable P700 and the maximal photochemical efficiency of PSII (Fv/Fm) in transgenic plants decreased more slowly at chilling temperature under low irradiance. These results suggested that suppression of LeZE caused zeaxanthin accumulation, which was helpful in alleviating photoinhibition of PSI and PSII in tomato plants under chilling stress.

Additional key words

antisense transgenic plants chilling stress under low irradiance photoinhibition tomato zeaxanthin epoxidase gene. 

Abbreviations

A

antheraxanthin

Chl

chlorophyll

Fm

maximum yield of fluorescence in darkness

Fm

maximum yield of fluorescence during illumination

Fo

initial fluorescence

Fv

variable fluorescence

Fv/Fm

maximal photochemical efficiency of PSII

HPLC

high performance liquid chromatography

LeZE

Lycopersicon esculentum zeaxanthin epoxidase gene

NADPH

nicotinamide adenine dinucleotide phosphate

NPQ

non-photochemical quenching

PPFD

photosynthetic photon flux density

PSI

photosystem I

PSII

photosystem II

ROS

reactive oxygen species

V

violaxanthin

VDE

violaxanthin de-epoxidase

Z

zeaxanthin

ZE

zeaxanthin epoxidase

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • N. Wang
    • 1
  • B. Li
    • 1
  • H. -L. Feng
    • 1
  • Q. -Y. Zhang
    • 1
  • X. -H. Yang
    • 1
  • Q. -W. Meng
    • 1
  1. 1.College of Life Science, State Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anP.R. China

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