Aerosolized Non-viral Nucleic Acid Delivery in the Vaginal Tract of Pigs
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- Remaut, K., De Clercq, E., Andries, O. et al. Pharm Res (2016) 33: 384. doi:10.1007/s11095-015-1796-x
The human pathogen Chlamydia trachomatis is worldwide the leading cause of bacterial sexually transmitted disease. Nasal or vaginal nucleic acid vaccination is a promising strategy for controlling genital Chlamydia trachomatis infections. Since naked nucleic acids are generally not efficiently taken up by cells, they are often complexed with carriers that facilitate their intracellular delivery.
In the current study, we screened a variety of commonly used non-viral gene delivery carriers for their ability to transfect newborn pig tracheal cells. The effect of aerosolization on the physicochemical properties and transfection efficiency of the complexes was also evaluated in vitro. Subsequently, a pilot experiment was performed in which the selected complexes were aerosolized in the vaginal tract of pigs.
Both mRNA and pDNA containing lipofectamine and ADM70 complexes showed promise for protein expression in vitro, before and after aerosolization. In vivo, only lipofectamine/pDNA complexes resulted in high protein expression levels 24 h following aerosolization. This correlates to the unexpected observation that the presence of vaginal mucus increases the efficiency of lipofectamine/pDNA complexes 3-fold, while the efficiency of lipofectamine/mRNA complexes and ADM70/mRNA and ADM70/pDNA complexes decreased.
As aerosolization was an easy and effective method to deliver complexes to the vaginal tract of pigs, we believe this application technique has future potential for both vaginal and perhaps nasal vaccination using non-viral gene delivery vectors.
Key wordsplasmid DNAmRNAaerosolizationvaginal administrationnon-viral gene delivery complexes
Dynamic light scattering
Dulbecco’s modified Eagle’s medium
Fetal calf serum
Green fluorescent protein
Genzyme lipid 67
3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide
Newborn pig tracheal
Regions of interest