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Bone Morphogenetic Protein-2 Release from Composite Hydrogels of Oligo(poly(ethylene glycol) fumarate) and Gelatin

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ABSTRACT

Purpose

Hydrogel composites of oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles (GMs) were investigated as carriers of bone morphogenetic protein-2 (BMP-2) for bone tissue engineering applications.

Methods

Hydrogel composites with different physical characteristics were prepared by changing the amount and type (acidic vs. basic) of gelatin incorporated in the OPF bulk phase. Composites with differing physical properties (degradation, swelling, and mechanical properties) and differing BMP-2 loading phase were investigated to determine the effect of these factors on BMP-2 release profiles over 28 days.

Results

Overall, higher gelatin amount increased the degradation and swelling of composites, and acidic GMs further increased the degradation and swelling and reduced the compressive modulus of the composites. The most significant factor affecting the release of BMP-2 from composites was the loading phase of the growth factor: GM loading reduced the burst release, increased BMP-2 release during the later phases of the experiment, and increased the cumulative release in faster degrading samples.

Conclusions

The results indicate that the physical properties and the BMP-2 release kinetics of hydrogel composites can be controlled by adjusting multiple parameters at the time of the hydrogel composite fabrication.

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Abbreviations

BMP-2:

Bone morphogenetic protein-2

col-PBS:

PBS containing 400 ng/ml collagenase type 1A

GM:

Gelatin microparticle

HA:

High acidic group

HB:

High basic group

LA:

Low acidic group

LB:

Low basic group

OPF:

Oligo(poly(ethylene glycol) fumarate)

pI:

Isoelectric point

xxG:

Gelatin loaded BMP-2

xxO:

OPF loaded BMP-2

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ACKNOWLEDGMENTS AND DISCLOSURES

The research described in this manuscript was supported by a grant from the Armed Forces Institute of Regenerative Medicine (W81XWH-08-2-0032). L.A.K. acknowledges support from a graduate fellowship from the National Science Foundation (0940902).

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas, USA

    Lucas A. Kinard & Antonios G. Mikos

  2. Department of Bioengineering - MS142, Rice University, P.O. Box 1892, Houston, Texas, 77251-1892, USA

    Chia-Ye Chu, F. Kurtis Kasper & Antonios G. Mikos

  3. Department of Biomaterials, Kyoto University, Kyoto, Japan

    Yasuhiko Tabata

Authors
  1. Lucas A. Kinard
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  2. Chia-Ye Chu
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  3. Yasuhiko Tabata
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  5. Antonios G. Mikos
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Correspondence to Antonios G. Mikos.

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Kinard, L.A., Chu, CY., Tabata, Y. et al. Bone Morphogenetic Protein-2 Release from Composite Hydrogels of Oligo(poly(ethylene glycol) fumarate) and Gelatin. Pharm Res 30, 2332–2343 (2013). https://doi.org/10.1007/s11095-013-1077-5

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  • DOI: https://doi.org/10.1007/s11095-013-1077-5

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