In Vivo Investigation of Hybrid Paclitaxel Nanocrystals with Dual Fluorescent Probes for Cancer Theranostics
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To develop novel hybrid paclitaxel (PTX) nanocrystals, in which bioactivatable (MMPSense® 750 FAST) and near infrared (Flamma Fluor® FPR-648) fluorophores are physically incorporated, and to evaluate their anticancer efficacy and diagnostic properties in breast cancer xenograft murine model.
The pure and hybrid paclitaxel nanocrystals were prepared by an anti-solvent method, and their physical properties were characterized. The tumor volume change and body weight change were evaluated to assess the treatment efficacy and toxicity. Bioimaging of treated mice was obtained non-invasively in vivo.
The released MMPSense molecules from the hybrid nanocrystals were activated by matrix metalloproteinases (MMPs) in vivo, similarly to the free MMPSense, demonstrating its ability to monitor cancer progression. Concurrently, the entrapped FPR-648 was imaged at a different wavelength. Furthermore, when administered at 20 mg/kg, the nanocrystal formulations exerted comparable efficacy as Taxol®, but with decreased toxicity.
Hybrid nanocrystals that physically integrated two fluorophores were successfully prepared from solution. Hybrid nanocrystals were shown not only exerting antitumor activity, but also demonstrating the potential of multi-modular bioimaging for diagnostics.
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- In Vivo Investigation of Hybrid Paclitaxel Nanocrystals with Dual Fluorescent Probes for Cancer Theranostics
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- breast cancer
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- Author Affiliations
- 1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, 40536, USA
- 2. Biostatistics, Division of Cancer Biostatistics, Markey Cancer Center, University of Kentucky, Lexington, Kentucky, 40506, USA
- 3. Department of Surgery and Markey Cancer Center, University of Kentucky, Lexington, Kentucky, 40506, USA
- 4. Departments of Biopharmaceutical Sciences, Bioengineering, and Ophthalmology and Visual Sciences, University of Illinois, Chicago, Illinois, 60612, USA
- 5. Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, Heine Pharmacy Building, 575 Stadium Mall Drive, West Lafayette, Indiana, 47907-2091, USA