Pharmaceutical Research

, Volume 29, Issue 12, pp 3393–3403

Multi-Compartmental Vaccine Delivery System for Enhanced Immune Response to gp100 Peptide Antigen in Melanoma Immunotherapy

  • Mayurkumar Kalariya
  • Srinivas Ganta
  • Mansoor Amiji
Research Paper

DOI: 10.1007/s11095-012-0834-1

Cite this article as:
Kalariya, M., Ganta, S. & Amiji, M. Pharm Res (2012) 29: 3393. doi:10.1007/s11095-012-0834-1

Abstract

Purpose

To develop a multi-compartmental vaccine delivery system for safe and efficient delivery of the gp100 peptide antigen in melanoma immunotherapy.

Methods

Water-in-oil-in-water (W/O/W) multiple emulsion-based multi-compartmental vaccine delivery system containing the gp100 peptide was prepared by a two-step emulsification method. In vivo prophylactic and active immunization effectiveness of the novel squalane oil-containing gp100 vaccine was evaluated in the murine B16 melanoma model and compared with that of an incomplete Freund’s adjuvant (IFA)-based vaccine.

Results

Morphological evaluation of the W/O/W multiple emulsions showed that the oil-droplets were homogenously dispersed with the gp100 peptide encapsulated in an inner aqueous phase. Immunization with the gp100 peptide delivered in the W/O/W multiple emulsions-based vaccine resulted in increased protection against tumor challenge compared to IFA-based vaccine (p < 0.05, n = 8) signifying induction of enhanced anti-tumor immunity. In addition, serum Th1 cytokine levels and immuno-histochemistry of excised tumor tissues indicated activation and local infiltration of antigen specific cytotoxic T-lymphocytes into and/or surrounding the tumor mass. Moreover, the newly developed vaccine formulation did not induce any overt systemic toxicity.

Conclusion

Novel W/O/W multiple emulsions-based vaccine efficiently delivers the gp100 peptide antigen to induce cell-mediated anti-tumor immunity and offers an alternate, safe vaccine delivery system.

KEY WORDS

anti-tumor immunitycancer vaccinegp100 peptide antigenmelanomaW/O/W multiple emulsions

Abbreviations

APCs

antigen-presenting cells

CTLs

cytotoxic T-lymphocytes

IFA

incomplete Freund’s adjuvant

PBS

phosphate-buffered saline

SME

squalane oil multiple emulsions

W/O/W

water-in-oil-in-water

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mayurkumar Kalariya
    • 1
  • Srinivas Ganta
    • 2
  • Mansoor Amiji
    • 1
  1. 1.Department of Pharmaceutical Sciences, School of PharmacyNortheastern UniversityBostonUSA
  2. 2.Nemucore Medical Innovations, Inc.WorcesterUSA