Pharmaceutical Research

, Volume 29, Issue 5, pp 1203–1218

Magnetic Nanoparticles Enhance Adenovirus Transduction In Vitro and In Vivo

Authors

    • OZ BiosciencesParc Scientifique de Luminy
  • Christophe Pellegrino
    • INSERM UMR S901, Institut de Neurobiologie de la Méditerranée (INMED)Parc Scientifique de Luminy
  • Nicolas Laurent
    • OZ BiosciencesParc Scientifique de Luminy
  • Flavie Sicard
    • OZ BiosciencesParc Scientifique de Luminy
    • OZ BiosciencesParc Scientifique de Luminy
Research Paper

DOI: 10.1007/s11095-011-0629-9

Cite this article as:
Sapet, C., Pellegrino, C., Laurent, N. et al. Pharm Res (2012) 29: 1203. doi:10.1007/s11095-011-0629-9

ABSTRACT

Purpose

Adenoviruses are among the most powerful gene delivery systems. Even if they present low potential for oncogenesis, there is still a need for minimizing widespread delivery to avoid deleterious reactions. In this study, we investigated Magnetofection efficiency to concentrate and guide vectors for an improved targeted delivery.

Method

Magnetic nanoparticles formulations were complexed to a replication defective Adenovirus and were used to transduce cells both in vitro and in vivo. A new integrated magnetic procedure for cell sorting and genetic modification (i-MICST) was also investigated.

Results

Magnetic nanoparticles enhanced viral transduction efficiency and protein expression in a dose-dependent manner. They accelerated the transduction kinetics and allowed non-permissive cells infection. Magnetofection greatly improved adenovirus-mediated DNA delivery in vivo and provided a magnetic targeting. The i-MICST results established the efficiency of magnetic nanoparticles assisted viral transduction within cell sorting columns.

Conclusion

The results showed that the combination of Magnetofection and Adenoviruses represents a promising strategy for gene therapy. Recently, a new integrated method to combine clinically approved magnetic cell isolation devices and genetic modification was developed. In this study, we validated that magnetic cell separation and adenoviral transduction can be accomplished in one reliable integrated and safe system.

KEY WORDS

AdenoMagadenovirusin vivo transductionmagnetic cell sortingmagnetofection

Copyright information

© Springer Science+Business Media, LLC 2011