Research Paper

Pharmaceutical Research

, Volume 29, Issue 2, pp 362-374

Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles

  • Won Ho KongAffiliated withDepartment of Biological Sciences, Graduate School of Nanoscience & Technology Korea Advanced Institute of Science & TechnologyDepartment of Material Science & Engineering, Pohang University of Science & Technology Email author 
  • , Ki Hyun BaeAffiliated withDepartment of Biological Sciences, Graduate School of Nanoscience & Technology Korea Advanced Institute of Science & Technology
  • , Sung Duk JoAffiliated withDepartment of Biological Sciences, Graduate School of Nanoscience & Technology Korea Advanced Institute of Science & Technology
  • , Jee Seon KimAffiliated withDepartment of Biological Sciences, Graduate School of Nanoscience & Technology Korea Advanced Institute of Science & Technology
  • , Tae Gwan ParkAffiliated withDepartment of Biological Sciences, Graduate School of Nanoscience & Technology Korea Advanced Institute of Science & Technology

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ABSTRACT

Purpose

Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA.

Methods

Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines.

Results

Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine.

Conclusions

We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications.

KEY WORDS

cancer therapy cationic lipid delivery system gold nanoparticle siRNA