Pharmaceutical Research

, Volume 29, Issue 1, pp 97–109

Solid Lipid Nanoparticles Loaded with Anti-microRNA Oligonucleotides (AMOs) for Suppression of MicroRNA-21 Functions in Human Lung Cancer Cells

Research Paper

DOI: 10.1007/s11095-011-0514-6

Cite this article as:
Shi, SJ., Zhong, ZR., Liu, J. et al. Pharm Res (2012) 29: 97. doi:10.1007/s11095-011-0514-6

ABSTRACT

Purpose

Literature has highlighted the practical use of solid lipid nanoparticles (SLNs) in research, but few reports have combined SLNs with miRNA-based therapy. We aimed to prepare SLNs to load anti-miRNA oligonucleotide (AMO) for miRNA-based therapy in vitro.

Methods

SLNs were employed to encapsulate AMO by a solvent diffusion method, and then the properties of AMO-CLOSs (cationic lipid binded oligonucleotide (AMO)-loaded SLNs) were characterized. We studied cellular uptake and activation properties of AMO-CLOSs in A549 cells, including antisense efficiency, cell migration and invasion.

Results

AMO-CLOSs were 187 nm in size and 46.6 mV in zeta potential with an approximately toroid morphology in the TEM image. AMO-CLOSs uptake by A549 cells was increased significantly higher and more effective than free AMO. Further results demonstrated that AMO-CLOSs showed high antisense efficiency of microRNA-21 and subsequently decreased the proliferation, migration and invasion of tumor cells.

Conclusions

These findings suggest that AMO-CLOSs represent a potential new approach for carrying anti-miRNA inhibitors for cancer therapy.

KEY WORDS

anti-miRNA oligonucleotide (AMO) antisense efficiency microRNA-21 microRNA-based therapy solid lipid nanoparticles 

ABBREVIATIONS

AMO

anti-miRNA oligonucleotide

AMO-CLOSs

cationic lipid binded oligonucleotide-loaded SLNs

DDAB-AMO

DDAB-binded AMO complexes

miR-21

microRNA-21

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education, West China School of PharmacySichuan UniversityChengduPeople’s Republic of China