Pharmaceutical Research

, Volume 28, Issue 6, pp 1395–1405

Chondrogenic Priming Adipose-Mesenchymal Stem Cells for Cartilage Tissue Regeneration

  • Nathaniel S. Hwang
  • Sung Gap Im
  • Patrick B. Wu
  • David A. Bichara
  • Xing Zhao
  • Mark A. Randolph
  • Robert Langer
  • Daniel G. Anderson
Research Paper

DOI: 10.1007/s11095-011-0445-2

Cite this article as:
Hwang, N.S., Im, S.G., Wu, P.B. et al. Pharm Res (2011) 28: 1395. doi:10.1007/s11095-011-0445-2

ABSTRACT

Purpose

Chondrocytes lose their ability to produce cartilaginous matrix during multiplication in culture through repeated passages, resulting in inferior tissue phenotype. To overcome the limited amount of primary chondrocytes, we aimed to determine the optimal culture condition for in vitro/in vivo cartilage regeneration using human adipose-derived mesenchymal stem cells (AMSCs).

Methods

To evaluate the effects exerted by the chondrocytic culture condition on AMSC, we utilized chondrocyte conditioned medium (CM) and/or co-culture methods to prime and differentiate AMSCs. We evaluated ultimate in vivo engineered cartilage with primed AMSCs with that of chondrocytes. To examine the link between conditioned factors and proliferation/differentiation, cell cycle progression of AMSCs were examined using 5-ethynyl-2′-deoxyuridine (EdU), and gene expression was monitored.

Results

We report that AMSCs can be stimulated to become chondrogenic cells when expanded with chondrocyte CM. Polymeric scaffolds co-seeded with CM- expanded AMSCs and primary chondrocytes resulted in in vivo cartilaginous tissues with similar biochemical content to constructs seeded with chondrocytes alone.

Conclusion

These results indicate that chondrocyte CM consists of suitable morphogenetic factors that induce the chondrogenic priming of AMSCs for cartilage tissue engineering.

KEY WORDS

auricular chondrocytescartilageco-culturehuman adipose derived mesenchymal stem cellstissue engineering

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Nathaniel S. Hwang
    • 1
  • Sung Gap Im
    • 2
    • 3
  • Patrick B. Wu
    • 1
  • David A. Bichara
    • 4
  • Xing Zhao
    • 4
  • Mark A. Randolph
    • 4
  • Robert Langer
    • 1
    • 2
  • Daniel G. Anderson
    • 1
    • 2
  1. 1.David H. Koch Institute for Integrative Cancer ResearchMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Department of Chemical EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  3. 3.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea
  4. 4.Plastic Surgery Research LaboratoryMassachusetts General Hospital, Harvard Medical SchoolBostonUSA