Pharmaceutical Research

, Volume 28, Issue 2, pp 279–291

An Effective Strategy for the Synthesis of Biocompatible Gold Nanoparticles Using Cinnamon Phytochemicals for Phantom CT Imaging and Photoacoustic Detection of Cancerous Cells

Authors

    • Department of RadiologyUniversity of Missouri
  • Ravi Shukla
    • Department of RadiologyUniversity of Missouri
  • Ajit Zambre
    • Department of RadiologyUniversity of Missouri
  • Swapna Mekapothula
    • Department of RadiologyUniversity of Missouri
  • Rajesh R. Kulkarni
    • Department of RadiologyUniversity of Missouri
  • Kavita Katti
    • Department of RadiologyUniversity of Missouri
  • Kiran Bhattacharyya
    • Department of Biological EngineeringUniversity of Missouri
  • Genevieve M. Fent
    • Department of Veterinary PathobiologyUniversity of Missouri
  • Stan W. Casteel
    • Department of Veterinary PathobiologyUniversity of Missouri
  • Evan J. Boote
    • Department of RadiologyUniversity of Missouri
  • John A. Viator
    • Department of Biological EngineeringUniversity of Missouri
  • Anandhi Upendran
    • Nanoparticle Biochem, Inc.
  • Raghuraman Kannan
    • Department of RadiologyUniversity of Missouri
  • Kattesh V. Katti
    • Department of RadiologyUniversity of Missouri
Research Paper

DOI: 10.1007/s11095-010-0276-6

Cite this article as:
Chanda, N., Shukla, R., Zambre, A. et al. Pharm Res (2011) 28: 279. doi:10.1007/s11095-010-0276-6

ABSTRACT

Purpose

The purpose of the present study was to explore the utilization of cinnamon-coated gold nanoparticles (Cin-AuNPs) as CT/optical contrast-enhancement agents for detection of cancer cells.

Methods

Cin-AuNPs were synthesized by a “green” procedure, and the detailed characterization was performed by physico-chemical analysis. Cytotoxicity and cellular uptake studies were carried out in normal human fibroblast and cancerous (PC-3 and MCF-7) cells, respectively. The efficacy of detecting cancerous cells was monitored using a photoacoustic technique. In vivo biodistribution was studied after IV injection of Cin-AuNPs in mice, and also a CT phantom model was generated.

Results

Biocompatible Cin-AuNPs were synthesized with high purity. Significant uptake of these gold nanoparticles was observed in PC-3 and MCF-7 cells. Cin-AuNPs internalized in cancerous cells facilitated detectable photoacoustic signals. In vivo biodistribution in normal mice showed steady accumulation of gold nanoparticles in lungs and rapid clearance from blood. Quantitative analysis of CT values in phantom model revealed that the cinnamon-phytochemical-coated AuNPs have reasonable attenuation efficiency.

Conclusions

The results indicate that these non-toxic Cin-AuNPs can serve as excellent CT/ photoacoustic contrast-enhancement agents and may provide a novel approach toward tumor detection through nanopharmaceuticals.

KEY WORDS

cancer cellscellular internalizationcinnamon-stabilized gold nanoparticlesin vivo biodistributionphantom CT imagingphotoacoustic detection

Copyright information

© Springer Science+Business Media, LLC 2010