Pharmaceutical Research

, Volume 27, Issue 6, pp 1138–1145

Multiple Berry Types Prevent N-nitrosomethylbenzylamine-Induced Esophageal Cancer in Rats

  • Gary D. Stoner
  • Li-Shu Wang
  • Claire Seguin
  • Claudio Rocha
  • Kristen Stoner
  • Steven Chiu
  • A. Douglas Kinghorn
Research Paper

DOI: 10.1007/s11095-010-0102-1

Cite this article as:
Stoner, G.D., Wang, LS., Seguin, C. et al. Pharm Res (2010) 27: 1138. doi:10.1007/s11095-010-0102-1

ABSTRACT

Purpose

The present study compared the ability of different berry types to prevent chemically-induced tumorigenesis in the rat esophagus. We also determined if berries influence the levels of inflammatory cytokines in the serum of carcinogen-treated rats.

Methods

Rats were treated with the carcinogen N-nitrosomethylbenzylamine (NMBA) for 5 weeks, then placed on diets containing 5% of either black or red raspberries, strawberries, blueberries, noni, açaí or wolfberry until the end of the study. The effects of the berries on tumor incidence, multiplicity and size were determined, as well as their effects on the levels of selected inflammatory cytokines in serum.

Results

All berry types were about equally effective in inhibiting NMBA-induced tumorigenesis in the rat esophagus. They also reduced the levels of the serum cytokines, interleukin 5 (IL-5) and GRO/KC, the rat homologue for human interleukin-8 (IL-8), and this was associated with increased serum antioxidant capacity.

Conclusions

Seven berry types were about equally capable of inhibiting tumor progression in the rat esophagus in spite of known differences in levels of anthocyanins and ellagitannins. Serum levels of IL-5 and GRO/KC (IL-8) may be predictive of the inhibitory effect of chemopreventive agents on rat esophageal carcinogenesis.

KEY WORDS

berries chemoprevention esophagus rat 

ABBREVIATIONS

AP-1

Activator protein-1

AIN-76A

American Institute of Nutrition-76A

BRBs

black raspberries

BBs

blueberries

DMSO

dimethylsulfoxide

EGF

epidermal growth factor

IL-8

GRO/KC

IFNγ

interferon-gamma

IL

interleukin

NMBA

N-nitrosomethylbenzylamine

RRBs

red raspberries

STRWs

strawberries

TPA

12-O-tetradecanolyphorbol-13-acetate

TNF-α

tumor necrosis factor-alpha

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Gary D. Stoner
    • 1
    • 3
  • Li-Shu Wang
    • 1
  • Claire Seguin
    • 1
  • Claudio Rocha
    • 1
  • Kristen Stoner
    • 1
  • Steven Chiu
    • 1
  • A. Douglas Kinghorn
    • 2
  1. 1.Department of Internal Medicine and Comprehensive Cancer Center, College of MedicineThe Ohio State UniversityColumbusUSA
  2. 2.Division of Medicinal Chemistry and Pharmacognosy, College of PharmacyThe Ohio State UniversityColumbusUSA
  3. 3.Department of Internal MedicineOhio State University Comprehensive Cancer CenterColumbusUSA

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