Research Paper

Pharmaceutical Research

, Volume 27, Issue 6, pp 1066-1071

First online:

Effect of Dietary Polyphenon E and EGCG on Lung Tumorigenesis in A/J Mice

  • Qi ZhangAffiliated withDepartment of Surgery and Alvin J Siteman Cancer Center, Washington University in St. Louis
  • , Huijing FuAffiliated withDepartment of Energy, Environmental and Chemical Engineering, Washington University in St Louis
  • , Jing PanAffiliated withDepartment of Surgery and Alvin J Siteman Cancer Center, Washington University in St. Louis
  • , Jun HeAffiliated withDepartment of Surgery and Alvin J Siteman Cancer Center, Washington University in St. Louis
  • , Seto RyotaAffiliated withMitsui Norin Co., Ltd
  • , Yukihiko HaraAffiliated withMitsui Norin Co., Ltd
  • , Yian WangAffiliated withDepartment of Surgery and Alvin J Siteman Cancer Center, Washington University in St. Louis
  • , Ronald A LubetAffiliated withChemoprevention Agent Development Research Group, National Cancer Institute
  • , Ming YouAffiliated withDepartment of Surgery and Alvin J Siteman Cancer Center, Washington University in St. Louis Email author 

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ABSTRACT

Purpose

To compare the chemopreventive efficacy of Polyphenon E (Poly E), (−)-epigallocatechin-3-gallate (EGCG) and Polyphenon E without EGCG (Poly E-EGCG) on the development of benzo(a)pyrene (B(a)P)-induced lung tumors in A/J mice.

Methods

Female A/J mice were given a single intraperitoneal injection of B(a)P (100 mg/kg body weight). One week after B(a)P injection, animals received AIN-76A purified powder diet containing 0.975% (wt/wt) EGCG, 0.525% (wt/wt) Poly E-EGCG or 1.5% (wt/wt) Poly E for 24 weeks or control diet with no additives.

Results

Poly E treatment significantly decreased tumor multiplicity by 52% and tumor load by 64%, while EGCG and Poly E-EGCG did not significantly inhibit lung tumor multiplicity. EGCG was more stable in a complex mixture (Poly E) than as a pure compound.

Conclusion

EGCG was ineffective when administered by diet likely due to its instability. Thus, EGCG’s efficacy on mice lung tumorigenesis requires the presence of other tea catechins.

KEY WORDS

chemoprevention degradation EGCG lung tumorigenesis polyphenon E