Pharmaceutical Research

, Volume 27, Issue 6, pp 1066–1071

Effect of Dietary Polyphenon E and EGCG on Lung Tumorigenesis in A/J Mice

  • Qi Zhang
  • Huijing Fu
  • Jing Pan
  • Jun He
  • Seto Ryota
  • Yukihiko Hara
  • Yian Wang
  • Ronald A Lubet
  • Ming You
Research Paper

DOI: 10.1007/s11095-010-0056-3

Cite this article as:
Zhang, Q., Fu, H., Pan, J. et al. Pharm Res (2010) 27: 1066. doi:10.1007/s11095-010-0056-3

ABSTRACT

Purpose

To compare the chemopreventive efficacy of Polyphenon E (Poly E), (−)-epigallocatechin-3-gallate (EGCG) and Polyphenon E without EGCG (Poly E-EGCG) on the development of benzo(a)pyrene (B(a)P)-induced lung tumors in A/J mice.

Methods

Female A/J mice were given a single intraperitoneal injection of B(a)P (100 mg/kg body weight). One week after B(a)P injection, animals received AIN-76A purified powder diet containing 0.975% (wt/wt) EGCG, 0.525% (wt/wt) Poly E-EGCG or 1.5% (wt/wt) Poly E for 24 weeks or control diet with no additives.

Results

Poly E treatment significantly decreased tumor multiplicity by 52% and tumor load by 64%, while EGCG and Poly E-EGCG did not significantly inhibit lung tumor multiplicity. EGCG was more stable in a complex mixture (Poly E) than as a pure compound.

Conclusion

EGCG was ineffective when administered by diet likely due to its instability. Thus, EGCG’s efficacy on mice lung tumorigenesis requires the presence of other tea catechins.

KEY WORDS

chemopreventiondegradationEGCGlung tumorigenesispolyphenon E

Supplementary material

11095_2010_56_MOESM1_ESM.doc (33 kb)
Supplementary Table 1Components of Poly E-EGCG and Poly E (DOC 33 kb)

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Qi Zhang
    • 1
  • Huijing Fu
    • 2
  • Jing Pan
    • 1
  • Jun He
    • 1
  • Seto Ryota
    • 3
  • Yukihiko Hara
    • 3
  • Yian Wang
    • 1
  • Ronald A Lubet
    • 4
  • Ming You
    • 1
  1. 1.Department of Surgery and Alvin J Siteman Cancer CenterWashington University in St. LouisSt LouisUSA
  2. 2.Department of Energy, Environmental and Chemical EngineeringWashington University in St LouisSt. LouisUSA
  3. 3.Mitsui Norin Co., LtdShizuokaJapan
  4. 4.Chemoprevention Agent Development Research GroupNational Cancer InstituteBethesdaUSA