Biantennary Glycans as Well as Genetic Variants of α1-Acid Glycoprotein Control the Enantioselectivity and Binding Affinity of Oxybutynin
- Tomoko KimuraAffiliated withGraduate School of Pharmaceutical Sciences, Kyoto University
- , Akimasa ShibukawaAffiliated withFaculty of Pharmaceutical Sciences, Chiba Institute of Science
- , Katsumi MatsuzakiAffiliated withGraduate School of Pharmaceutical Sciences, Kyoto University Email author
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The purpose of this study was to investigate the role of biantennary branching glycans of α1-acid glycoprotein (AGP) and its genetic variants in the enantioselective binding of oxybutynin (OXY).
Human native AGP was separated using imminodiacetate–copper (II) affinity chromatography into two fractions, the A variant and a mixture of the F1 and S variants (F1–S). These fractionated AGPs were further separated by concanavalin A affinity chromatography into two fractions, with and without biantenarry glycans. An on-line high-performance liquid chromatography (HPLC) system consisting of a high-performance frontal analysis column, an extraction column, and an analytical HPLC column was developed to determine the binding affinities of OXY enantiomers for respective AGP species.
The total binding affinity as well as the enantiomeric selectivity of OXY in the F1–S mixed variant was significantly higher than that for the A variant, indicating that the chiral recognition ability of native AGP for the OXY enantiomers highly depends on the F1–S mixed variant. Furthermore, not only the genetic variants but also bianntenary glycans of AGP affect the binding affinity of OXY and are also responsible for the enantioselectivity.
Both genetic variants and glycan structures significantly contribute to the enantioselectivity and the binding affinity of OXY.
Key Wordsα1-acid glycoprotein biantennary branching glycan genetic variants high performance frontal analysis oxybutynin protein binding sugar chain
- Biantennary Glycans as Well as Genetic Variants of α1-Acid Glycoprotein Control the Enantioselectivity and Binding Affinity of Oxybutynin
Volume 23, Issue 5 , pp 1038-1042
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- α1-acid glycoprotein
- biantennary branching glycan
- genetic variants
- high performance frontal analysis
- protein binding
- sugar chain
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