Origins of Life and Evolution of Biospheres

, Volume 43, Issue 4, pp 411–428

The Possible Interplanetary Transfer of Microbes: Assessing the Viability of Deinococcus spp. Under the ISS Environmental Conditions for Performing Exposure Experiments of Microbes in the Tanpopo Mission

  • Yuko Kawaguchi
  • Yinjie Yang
  • Narutoshi Kawashiri
  • Keisuke Shiraishi
  • Masako Takasu
  • Issay Narumi
  • Katsuya Satoh
  • Hirofumi Hashimoto
  • Kazumichi Nakagawa
  • Yoshiaki Tanigawa
  • Yoh-hei Momoki
  • Maiko Tanabe
  • Tomohiro Sugino
  • Yuta Takahashi
  • Yasuyuki Shimizu
  • Satoshi Yoshida
  • Kensei Kobayashi
  • Shin-ichi Yokobori
  • Akihiko Yamagishi
Astrobiology

DOI: 10.1007/s11084-013-9346-1

Cite this article as:
Kawaguchi, Y., Yang, Y., Kawashiri, N. et al. Orig Life Evol Biosph (2013) 43: 411. doi:10.1007/s11084-013-9346-1

Abstract

To investigate the possible interplanetary transfer of life, numerous exposure experiments have been carried out on various microbes in space since the 1960s. In the Tanpopo mission, we have proposed to carry out experiments on capture and space exposure of microbes at the Exposure Facility of the Japanese Experimental Module of the International Space Station (ISS). Microbial candidates for the exposure experiments in space include Deinococcus spp.: Deinococcus radiodurans, D. aerius and D. aetherius. In this paper, we have examined the survivability of Deinococcus spp. under the environmental conditions in ISS in orbit (i.e., long exposure to heavy-ion beams, temperature cycles, vacuum and UV irradiation). A One-year dose of heavy-ion beam irradiation did not affect the viability of Deinococcus spp. within the detection limit. Vacuum (10−1 Pa) also had little effect on the cell viability. Experiments to test the effects of changes in temperature from 80 °C to −80 °C in 90 min (±80 °C/90 min cycle) or from 60 °C to −60 °C in 90 min (±60 °C/90 min cycle) on cell viability revealed that the survival rate decreased severely by the ±80 °C/90 min temperature cycle. Exposure of various thicknesses of deinococcal cell aggregates to UV radiation (172 nm and 254 nm, respectively) revealed that a few hundred micrometer thick aggregate of deinococcal cells would be able to withstand the solar UV radiation on ISS for 1 year. We concluded that aggregated deinococcal cells will survive the yearlong exposure experiments. We propose that microbial cells can aggregate as an ark for the interplanetary transfer of microbes, and we named it ‘massapanspermia’.

Keywords

PanspermiaSpace exposure experiments of microbesDeinococcusCell aggregationMassapanspermia

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yuko Kawaguchi
    • 1
  • Yinjie Yang
    • 1
    • 2
  • Narutoshi Kawashiri
    • 1
  • Keisuke Shiraishi
    • 1
  • Masako Takasu
    • 3
  • Issay Narumi
    • 4
    • 5
  • Katsuya Satoh
    • 4
  • Hirofumi Hashimoto
    • 6
  • Kazumichi Nakagawa
    • 7
  • Yoshiaki Tanigawa
    • 7
  • Yoh-hei Momoki
    • 7
  • Maiko Tanabe
    • 7
  • Tomohiro Sugino
    • 1
  • Yuta Takahashi
    • 1
  • Yasuyuki Shimizu
    • 1
  • Satoshi Yoshida
    • 8
  • Kensei Kobayashi
    • 9
  • Shin-ichi Yokobori
    • 1
  • Akihiko Yamagishi
    • 1
  1. 1.Laboratory for Extremophiles, Department of Applied Molecular Biology, School of Life SciencesTokyo University of Pharmacy and Life SciencesTokyoJapan
  2. 2.Protein Metabolism ProjectTokyo Metropolitan Institute of Medical ScienceTokyoJapan
  3. 3.Computational Biophysics Laboratory, Department of Molecular Biology, School of Life SciencesTokyo University of Pharmacy and Life SciencesTokyoJapan
  4. 4.Ion Beam Mutagenesis Research Group, Quantum Beam Science Directorate, Japan Atomic Energy AgencyTakasakiJapan
  5. 5.Graduate school of Life SciencesToyo UniversityOra-gunJapan
  6. 6.Institute of Space and Astronautical Science, JAXASagamiharaJapan
  7. 7.Graduate School of Human Development and EnvironmentKobe UniversityKobeJapan
  8. 8.National Institute of Radiological SciencesChibaJapan
  9. 9.Graduate School of EngineeringYokohama National UniversityHodogaya-kuJapan