Optical and Quantum Electronics

, Volume 37, Issue 13, pp 1397–1408

Tomographic Imaging of Collagen Fiber Orientation in Human Tissue Using Depth-Resolved Polarimetry of Second-Harmonic-Generation Light

  • Takeshi Yasui
  • Kunihiko Sasaki
  • Yoshiyuki Tohno
  • Tsutomu Araki
Article

DOI: 10.1007/s11082-005-4219-0

Cite this article as:
Yasui, T., Sasaki, K., Tohno, Y. et al. Opt Quant Electron (2005) 37: 1397. doi:10.1007/s11082-005-4219-0

Abstract

We propose a nonlinear optical probe method to image the distribution of collagen fiber orientation in human tissue by measuring the polarization of collagen-induced second-harmonic-generation (SHG) light (SHG polarimetry). Depth-resolved SHG polarimetry, with a depth resolution of 14 μm, was used to evaluate the cross-sectional profile of collagen fiber orientation in Achilles tendon and dentin, revealing a characteristic distribution of collagen orientation along the depth direction. We evaluated the two-dimensional (2D) lateral distribution of collagen fiber orientation in back reticular dermis and anklebone by polarization-resolved SHG imaging, and confirmed an appreciable difference in the distribution profiles of the two samples. We further extended the method to a depth-resolved measurement of the three-dimensional (3D) distribution of collagen orientation in anklebone. The proposed system promises to be a powerful tool for in vivo measurement of collagen fiber orientation in human tissue.

Keywords

collagenfemtosecond laserorientationpolarimetrysecond-harmonic-generationtomography

Copyright information

© Springer 2006

Authors and Affiliations

  • Takeshi Yasui
    • 1
  • Kunihiko Sasaki
    • 1
  • Yoshiyuki Tohno
    • 2
  • Tsutomu Araki
    • 1
  1. 1.Graduate School of Engineering ScienceOsaka UniversityToyonakaJapan
  2. 2.1st Department of AnatomyNara Medical UniversityKashiharaJapan