Original Paper

Neurochemical Research

, Volume 37, Issue 12, pp 2706-2714

First online:

Effect of One-Year Vitamin C- and E-Supplementation on Cerebrospinal Fluid Oxidation Parameters and Clinical Course in Alzheimer’s Disease

  • Sönke ArltAffiliated withDepartment of Psychiatry and Psychotherapy, University Medical Center Hamburg-Eppendorf Email author 
  • , Tomas Müller-ThomsenAffiliated withDepartment of Psychiatry and Psychotherapy, University Medical Center Hamburg-Eppendorf
  • , Ulrike BeisiegelAffiliated withInstitute for Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf
  • , Anatol KontushAffiliated withDyslipidemia, Inflammation and Atherosclerosis Research Unit (UMRS 939), National Institute for Health and Medical Research (INSERM)

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Abstract

Antioxidant vitamins are being widely discussed as a therapeutic option in Alzheimer’s disease (AD). We recently found that supplementation with vitamin C and E over 1 month leads to an increase of their levels in cerebrospinal fluid (CSF) and a reduction of CSF lipid peroxidation. In the present study, we followed-up the biochemical and clinical effect of vitamin C and E supplementation in an open clinical trial over 1 year. Twelve AD patients stably taking a cholinesterase inhibitor were supplemented with vitamin C (1,000 mg/day) and E (400 I.U./day), while 11 patients taking cholinergic medication only served as a control group. Cognition was assessed at baseline, after 6 months and 12 months using the Mini-Mental State Examination; a more detailed testing of cognitive function was performed at baseline and after 12 months. From eight of the vitamin-supplemented patients, CSF was taken at baseline, after 1 month and after 1 year to measure the antioxidant effect of vitamin supplementation on CSF lipids using a recently established in vitro oxidation assay. CSF antioxidant vitamins were significantly increased after 1 month and 1 year of supplementation, while in vitro oxidation of CSF lipids was significantly reduced only after 1 year of the supplementation. The clinical course of AD did not significantly differ between the vitamin and the control group. We conclude that supplementation with vitamins E and C did not have a significant effect on the course of AD over 1 year despite of a limited antioxidant effect that could be observed in CSF.

Keywords

Alzheimer’s disease Cerebrospinal fluid Vitamin E, vitamin C Oxidative stress