Neurochemical Research

, Volume 37, Issue 11, pp 2562–2568

Significance of Short Chain Fatty Acid Transport by Members of the Monocarboxylate Transporter Family (MCT)

  • Ivano Moschen
  • Angelika Bröer
  • Sandra Galić
  • Florian Lang
  • Stefan Bröer
Original Paper

DOI: 10.1007/s11064-012-0857-3

Cite this article as:
Moschen, I., Bröer, A., Galić, S. et al. Neurochem Res (2012) 37: 2562. doi:10.1007/s11064-012-0857-3

Abstract

Metabolism of short-chain fatty acids (SCFA) in the brain, particularly that of acetate, appears to occur mainly in astrocytes. The differential use has been attributed to transport, but the extent to which transmembrane movement of SCFA is mediated by transporters has not been investigated systematically. Here we tested the possible contribution of monocarboxylate transporters to SCFA uptake by measuring fluxes with labelled compounds and by following changes of the intracellular pH in Xenopus laevis oocytes expressing the isoforms MCT1, MCT2 or MCT4. All isoforms mediated significant transport of acetate. Formate, however, was transported only by MCT1. The contribution of MCT1 to SCFA transport was determined by using phloretin as a high-affinity inhibitor, which allowed a paired comparison of oocytes with and without active MCT1.

Keywords

AstrocytesAcetateShort-chain fatty acidsNon-ionic diffusion

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ivano Moschen
    • 2
    • 4
  • Angelika Bröer
    • 1
  • Sandra Galić
    • 1
    • 3
  • Florian Lang
    • 2
  • Stefan Bröer
    • 1
  1. 1.Research School of BiologyAustralian National UniversityCanberraAustralia
  2. 2.Physiologisches InstitutUniversity of TübingenTübingenGermany
  3. 3.St Vincents InstituteFitzroyAustralia
  4. 4.Department of Oral and Maxillo-Facial SurgeryUniversity of InnsbruckInnsbruckAustria